Immunosuppressive Property of Dried Human Amniotic Membrane

被引：13

作者：

Park, Choul Yong ^{[2
]}

Kohanim, Sahar ^{[1
]}

Zhu, Lei ^{[1
]}

Gehlbach, Peter L. ^{[1
]}

Chuck, Roy S. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Wilmer Ophthalmol Inst, Baltimore, MD 21287 USA

[2] Dongguk Univ, Dept Ophthalmol, Koyang, South Korea

来源：

OPHTHALMIC RESEARCH | 2009年 / 41卷 / 02期

关键词：

Amniotic membrane; T cell proliferation; Immune suppression; Cryopreservation; Ocular surface; EPITHELIAL-CELLS;

D O I：

10.1159/000187629

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay. Copyright (C) 2008 S. Karger AG, Basel

引用

页码：112 / 113

页数：2

共 9 条

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