Human alveolar epithelial cells type II are capable of regulating T-cell activity

被引:0
作者
Zissel, G
Ernst, M
Rabe, K
Papadopoulos, T
Magnussen, H
Schlaak, M
Müller-Quernheim, J
机构
[1] Hosp Med, Res Ctr, D-23845 Borstel, Germany
[2] Res Ctr, Dept Cell Biol, Borstel, Germany
[3] Krankenhaus Grosshansdorf, Grosshansdorf, Germany
[4] Univ Wurzburg, Dept Pathol, D-97070 Wurzburg, Germany
关键词
cell-to-cell interactions; costimulatory molecules; growth factors; human-clinical studies;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Alveolar epithelial cells type II (AEC-II) express MHC class II on their surface, an important prerequisite for antigen presentation. However, accessory signals are required for an efficient T-cell activation. We therefore isolated AEC-II from tumor-free sections of human lungs obtained by lobectomy/pneumectomy and purified the cells by magnetic-activated cell sorting. Furthermore, we tested the expression of CD54, CD58, CD80, and CD86 on AEC-II and evaluated their accessory function (AF) in cell culture using a coculture of interleukin-2 (IL-2), releasing Jurkat cells and AEC-II. An increased AF is documented by an elevated IL-2 release and expressed as accessory index (AI), In 33 experiments the AF of AEC-II proved to be highly variable. AI ranged between 0.3 and 17.1 with a median of 1.4 (0.3-17.1), Forty-four percent (4-77) of the AEC-II expressed HLA-DR, 44% (12-89%) of the cells expressed CD58, and CD54 was expressed by 55% (16-89%). AEC-II also expressed CD80 and CD86 (38% [0-77%] and 40% [4-68%], respectively), Interestingly, AEC-II released high levels of TGF beta (1730 pg/mL [771-5876]) and the accessory index could be increased (approximate to 2-fold) by the addition of neutralizing anti-TGF beta antibodies or radiation. Thus, type II alveolar cells express costimulatory molecules and are able to deliver costimulatory signals for T cells, providing evidence that AEC-II are able to act as antigen-presenting and immunoregulatory cells of the lung. Additionally, the accessory function of AEC-II is under the control of endogenously released TCF beta.
引用
收藏
页码:66 / 75
页数:10
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