Development and characterization of 15 microsatellite loci for Cariniana estrellensis and transferability to Cariniana legalis, two endangered tropical tree species

被引：12

作者：

Guidugli, Marcela Corbo ^{[1
]}

de Campos, Tatiana ^{[2
]}

Barbosa de Sousa, Adna Cristina ^{[2
]}

Feres, Juliana Massimino ^{[1
]}

Sebbenn, Alexandre Magno ^{[3
]}

Mestriner, Moacyr Antonio ^{[1
]}

Betioli Contel, Eucleia Primo ^{[1
]}

Alzate-Marin, Ana Lilia ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Lab Genet Vegetal, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Dept Genet & Evolucao, BR-13083970 Campinas, SP, Brazil

[3] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo, Brazil

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

Conservation genetics; Forest; Genetic diversity; Jequitiba branco; Molecular marker; SSR;

D O I：

10.1007/s10592-008-9672-4

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

From a genomic library enriched for GA/CA repeats, 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis, a tropical forest tree. The microsatellite loci were screened in 49 mature trees found between Pardo river and Mogi-Gua double dagger u river basins, in the state of So Paulo, Brazil. A total of 140 alleles were detected with an average of 9.33 alleles per locus. The expected heterozygosity ranged from 0.37 to 0.88. These loci showed a high probability of paternity exclusion. Additionally, 12 loci were effectively transferred to Cariniana legalis. High levels of polymorphism make the present SSR markers useful for population genetic studies.

引用

页码：1001 / 1004

页数：4

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