CM01: a facility for cryo-electron microscopy at the European Synchrotron

被引：73

作者：

Kandiah, Eaazhisai ^{[1
]}

Giraud, Thierry ^{[1
]}

Antolinos, Alejandro de Maria ^{[1
]}

Dobias, Fabien ^{[1
]}

Effantin, Gregory ^{[2
]}

Flot, David ^{[1
]}

Hons, Michael ^{[3
]}

Schoehn, Guy ^{[2
]}

Susini, Jean ^{[1
]}

Svensson, Olof ^{[1
]}

Leonard, Gordon A. ^{[1
]}

Mueller-Dieckmann, Christoph ^{[1
]}

机构：

[1] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38042 Grenoble, France

[2] Univ Grenoble Alpes, CNRS, CEA, IBS, 71 Ave Martyrs, F-38042 Grenoble, France

[3] Grenoble Outstn, European Mol Biol Lab, 71 Ave Martyrs, F-38042 Grenoble, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2019年 / 75卷

关键词：

cryo-TEM; ESRF; PSB; cryo-EM platform; CM01; MACROMOLECULAR CRYSTALLOGRAPHY BEAMLINES; CRYO-EM; SYSTEM; VISUALIZATION; SAXS;

D O I：

10.1107/S2059798319006880

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a `quantum leap' in the application of cryo-electron microscopy in structural biology, and many national and international centres have since been created in order to exploit this. Here, a new facility for cryo-electron microscopy focused on single-particle reconstruction of biological macromolecules that has been commissioned at the European Synchrotron Radiation Facility (ESRF) is presented. The facility is operated by a consortium of institutes co-located on the European Photon and Neutron Campus and is managed in a similar fashion to a synchrotron X-ray beamline. It has been open to the ESRF structural biology user community since November 2017 and will remain open during the 2019 ESRF-EBS shutdown.

引用

页码：528 / 535

页数：8

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