The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria

被引：58

作者：

Han, Jingluan ^{[1
,2
,3
]}

Wang, Xiaoyu ^{[1
,3
]}

Wang, Fengpin ^{[1
,3
]}

Zhao, Zhe ^{[1
,2
,3
]}

Li, Gousi ^{[1
,3
]}

Zhu, Xiaoyuan ^{[4
]}

Su, Jing ^{[4
]}

Chen, Letian ^{[1
,2
,3
]}

机构：

[1] South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agro B, Guangzhou 510642, Peoples R China

[2] South China Agr Univ, Guangdong Prov Key Lab Prot Funct & Regulat Agr O, Guangzhou 510642, Peoples R China

[3] South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China

[4] Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

来源：

RICE | 2021年 / 14卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Innate immunity; Effector; Avr-Pita; Mitochondrion; Cytochrome c oxidase (COX); Reactive oxygen species (ROS);

D O I：

10.1186/s12284-021-00453-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Background Avr-Pita was the first effector identified in the blast fungus (Magnaporthe oryzae)-rice (Oryza sativa) pathosystem. However, the molecular mechanism underlying its effects on the host plant has remained a long-standing mystery. Results Here, we report that ectopically expressing Avr-Pita in rice enhances susceptibility to M. oryzae and suppresses pathogen-associated molecular pattern (PAMP)-triggered defense responses. Avr-Pita targets the host mitochondria and interacts with the cytochrome c oxidase (COX) assembly protein OsCOX11, a key regulator of mitochondrial reactive oxygen species (ROS) metabolism in rice. Overexpressing Avr-Pita or OsCOX11 increased COX activity and decreased ROS accumulation triggered by the fungal PAMP chitin. OsCOX11-overexpressing plants showed increased susceptibility to M. oryzae, whereas OsCOX11-knockdown plants showed resistance to M. oryzae. Conclusions Taken together, these findings suggest that the fungal pathogen M. oryzae delivers the effector Avr-Pita to the host plant, where it enhances COX activity thus decreasing ROS accumulation. Therefore, this effector suppresses host innate immunity by perturbing ROS metabolism in the mitochondria.

引用

页数：11

共 45 条

[21] Higher plant mitochondria [J].