Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-β1-induced activation of kidney fibroblasts

被引：80
作者：
Zhu, Rong [1 ]
Zheng, Rong [1 ]
Deng, Yueyi [1 ]
Chen, Yiping [1 ]
Zhang, Shuwei [2 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Nephrol, Shanghai 200032, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China
来源：
PHYTOMEDICINE | 2014年 / 21卷 / 03期
关键词：
Ergosterol peroxide; Cordyceps cicadae; TGF-beta; 1; Chronic kidney disease; Fibroblast-to-myofibroblast transdifferentiaton; RENAL FIBROBLASTS; MAP KINASE; GROWTH; MYOFIBROBLASTS; RESPONSES; EXTRACTS; FIBROSIS; STEROLS; DISEASE; MATRIX;
D O I：
10.1016/j.phymed.2013.08.022
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Chronic kidney disease is a growing public health problem with an urgent need for new pharmacological agents. Ergosterol peroxide (EP) is the major sterol produced by Cordyceps cicadae Shing (C cicadae), a widely used traditional Chinese medicine. C cicadae has been used to treat many kinds of diseases and has a potential benefit on renoprotection. This study aimed to investigate the anti-fibrotic effects of EP as well as the underlying mechanisms. A normal rat kidney fibroblast cell line (NRK-49F) was stimulated to undergo fibroblast activation by transforming growth factor-beta 1 (TGF-beta 1) and EP treatment was applied to explore its potential anti-fibrotic effects. Cell proliferation was investigated using MTT analysis. Fibrosis-associated protein expression was analyzed using immunohistochemistry and/or Western blotting. EP treatment attenuated TGF-beta 1-induced renal fibroblast proliferation, expression of cytoskeleton protein and CTGF, as well as ECM production. Additionally, EP blocked TGF-beta 1-stimulated phosphorylation of ERK1/2, p38 and JNK pathway. Moreover, the TGF-beta 1-induced expression of fibronectin was attenuated by either inhibition of MAPKs or by EP treatment. In conclusion, our findings demonstrate that EP is able to suppress TGF-beta 1-induced fibroblasts activation in NRK-49F. This new information provides a line of theoretical evidence supporting the use of C cicadae in the intervention of kidney disease and suggests that EP has the potential to be developed as a therapeutic agent to prevent renal fibrosis. (C) 2013 Elsevier GmbH. All rights reserved.
引用
收藏
页码：372 / 378
页数：7
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