Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-β1-induced activation of kidney fibroblasts

被引：83

作者：

Zhu, Rong ^{[1
]}

Zheng, Rong ^{[1
]}

Deng, Yueyi ^{[1
]}

Chen, Yiping ^{[1
]}

Zhang, Shuwei ^{[2
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Nephrol, Shanghai 200032, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China

来源：

PHYTOMEDICINE | 2014年 / 21卷 / 03期

关键词：

Ergosterol peroxide; Cordyceps cicadae; TGF-beta; 1; Chronic kidney disease; Fibroblast-to-myofibroblast transdifferentiaton; RENAL FIBROBLASTS; MAP KINASE; GROWTH; MYOFIBROBLASTS; RESPONSES; EXTRACTS; FIBROSIS; STEROLS; DISEASE; MATRIX;

D O I：

10.1016/j.phymed.2013.08.022

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Chronic kidney disease is a growing public health problem with an urgent need for new pharmacological agents. Ergosterol peroxide (EP) is the major sterol produced by Cordyceps cicadae Shing (C cicadae), a widely used traditional Chinese medicine. C cicadae has been used to treat many kinds of diseases and has a potential benefit on renoprotection. This study aimed to investigate the anti-fibrotic effects of EP as well as the underlying mechanisms. A normal rat kidney fibroblast cell line (NRK-49F) was stimulated to undergo fibroblast activation by transforming growth factor-beta 1 (TGF-beta 1) and EP treatment was applied to explore its potential anti-fibrotic effects. Cell proliferation was investigated using MTT analysis. Fibrosis-associated protein expression was analyzed using immunohistochemistry and/or Western blotting. EP treatment attenuated TGF-beta 1-induced renal fibroblast proliferation, expression of cytoskeleton protein and CTGF, as well as ECM production. Additionally, EP blocked TGF-beta 1-stimulated phosphorylation of ERK1/2, p38 and JNK pathway. Moreover, the TGF-beta 1-induced expression of fibronectin was attenuated by either inhibition of MAPKs or by EP treatment. In conclusion, our findings demonstrate that EP is able to suppress TGF-beta 1-induced fibroblasts activation in NRK-49F. This new information provides a line of theoretical evidence supporting the use of C cicadae in the intervention of kidney disease and suggests that EP has the potential to be developed as a therapeutic agent to prevent renal fibrosis. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：372 / 378

页数：7

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