Ribonomic approaches to study the RNA-binding proteome

被引：39

作者：

Faoro, Camilla ^{[1
]}

Ataide, Sandro F. ^{[1
]}

机构：

[1] Univ Sydney, Sch Mol Biosci, Rm 672,Bldg G08, Sydney, NSW 2006, Australia

来源：

FEBS LETTERS | 2014年 / 588卷 / 20期

基金：

澳大利亚研究理事会;

关键词：

RNA-binding proteins (RBPs); Ribonucleoprotein particles (RNPs); Ribonome; RNA-protein interactions; RNA-centric methods; Non-coding RNAs (ncRNAs); AFFINITY PURIFICATION TECHNIQUE; SINGLE-NUCLEOTIDE RESOLUTION; CLASS SWITCH RECOMBINATION; ASH1; MESSENGER-RNA; IN-VITRO; RIBONUCLEOPROTEIN COMPLEXES; STRUCTURE PREDICTION; MASS-SPECTROMETRY; GLOBAL ANALYSIS; INTERACTING PROTEINS;

D O I：

10.1016/j.febslet.2014.07.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known or predicted RNA-binding domains. However, various methods have been recently developed to capture an RNA of interest and comprehensively identify its associated RBPs. In this review, we discuss the RNA-affinity purification methods followed by mass spectrometry analysis (AP-MS); RBP screening within protein libraries and computational methods that can be used to study the RNA-binding proteome (RBPome). (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3649 / 3664

页数：16

共 183 条

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