Mucosal vaccination with a multicomponent adenovirus-vectored vaccine protects against Streptococcus pneumoniae infection in the lung

被引:26
作者
Arevalo, Maria T. [1 ]
Xu, Qingfu [1 ]
Paton, James C. [2 ]
Hollingshead, Susan K. [3 ]
Pichichero, Michael E. [1 ]
Briles, David E. [3 ]
Girgis, Natasha [1 ]
Zeng, Mingtao [1 ]
机构
[1] Univ Rochester, Sch Med & Dent, Dept Microbiol & Immunol, Rochester, NY 14642 USA
[2] Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, Australia
[3] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2009年 / 55卷 / 03期
关键词
mucosal vaccination; pneumococcal vaccine; protective immunity; replication-defective adenovirus; Streptococcus pneumoniae; INTRANASAL IMMUNIZATION; PNEUMOCOCCAL PROTEINS; PULMONARY INFECTION; VIRULENCE PROTEINS; CONJUGATE VACCINE; MICE; PSPA; PNEUMOLYSIN; SYSTEM; PSAA;
D O I
10.1111/j.1574-695X.2008.00518.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Streptococcus pneumoniae is a major bacterial respiratory pathogen. Current licensed pneumococcal polysaccharide and polysaccharide-protein conjugate vaccines are administered by an intramuscular injection. In order to develop a new-generation vaccine that can be administered in a needle-free mucosal manner, we have constructed early 1 and 3 gene regions (E1/E3) deleted, replication-defective adenoviral vectors encoding pneumococcal surface antigen A (PsaA), the N-fragment of pneumococcal surface protein A (N-PspA), and the detoxified mutant pneumolysin (PdB) from S. pneumoniae strain D39. Intranasal vaccination with the three adenoviral vectors (Ad/PsaA, Ad/N-PspA, and Ad/PdB) in mice resulted in robust antigen-specific serum immunoglobulin G responses, as demonstrated by an enzyme-linked immunosorbent assay. In addition, nasal mucosal vaccination with the combination of the three adenoviral vectors conferred protection against S. pneumoniae strain D39 colonization in mouse lungs. Taken together, these data demonstrate the feasibility of developing a mucosal vaccine against S. pneumoniae using recombinant adenoviruses for antigen delivery.
引用
收藏
页码:346 / 351
页数:6
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