Ca2+-dependent inactivation of the class C L-type Ca2+ channel is a property of the alpha(1) subunit

被引：26
|
作者：
Zong, XG
Hofmann, F
机构：
[1] Inst. fur Pharmakol. und Toxikol., Tech. Universität München, 80802 München
来源：
FEBS LETTERS | 1996年 / 378卷 / 02期
关键词：
Ca2+-dependent inactivation; L-type Ca2+ channel; intracellular Ca2+ buffer; CHO cell; HEK; 293; cell;
D O I：
10.1016/0014-5793(95)01434-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stably expressed Ca2+ channel alpha(1C-a) and alpha(1C-b) subunit were used to investigate the molecular basis for Ca2+ dependent inactivation of the L-type current. The Ba2+ current (I-Ba) of both channels had similar kinetics and inactivated with one time constant of about 400 ms at + 20 mV whereas the Ca2+ current (I-Ca) could be fitted only with a bi-exponential function. The fast (tau(f)) and the slow (tau(s)) time constant were about 20 ms and 400 ms, respectively. The inactivation of I-Ca strongly depended on the entry of Ca2+ as shown by prepulses and variation of the intracellular Ca2+ chelator. Coexpression of the alpha(1C) subunits with the auxiliary alpha(2)/delta and beta subunits accelerated the voltage-dependent but not the Ca2+-dependent inactivation of the channels. These results suggest that the alpha(1C) subunit of L-type Ca2+ channels itself mediates the Ca2+-dependent inactivation of the current.
引用
收藏
页码：121 / 125
页数：5
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