SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement

被引：0

作者：

Yoshiki, K. ^{[1
,2
]}

Hashimoto, M. ^{[2
]}

Araki, T. ^{[2
]}

机构：

[1] Osaka Univ, Org Promot Res Nanosci & Nanotechnol, 1-3 Machikaneyama, Toyonaka, Osaka 5608531, Japan

[2] Osaka Univ, Grad Sch Engn Sci, Div Bioengn, Toyonaka, Osaka 5608531, Japan

来源：

LASER BEAM SHAPING VII | 2006年 / 6290卷

关键词：

second harmonic generation; molecular orientation measurement; spatial polarization control; spatial light modulator;

D O I：

10.1117/12.680506

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a second-harmonic-generation (SHG) microscope to observe the three-dimensional molecular orientation with three-dimensional high spatial resolution using a polarization mode converter. The mode converter consists of a parallel-aligned nematic-liquid-crystal spatial-light-modulator (PAL-SLM) and quarter-waveplates, and converts a incident linearly polarized beam to orthogonal linearly polarized beams or radially polarized beam. We combined the mode converter with SHG microscope to obtain the local information of the three-dimensional molecular orientation. We demonstrated the detection of three-dimensional molecular orientation of collagen fiber in human Achilles' tendon. For high precision three-dimensional molecular orientation measurement, we propose a technique to calibrate the dependence of SHG detection efficiencies on molecular orientation using a liposome.

引用

页数：8