Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

被引：15
作者：
Abdullah, MAF
Dean, DH
机构：
[1] Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA
[2] Int Islamic Univ Malaysia, Fac Engn, Dept Sci, Kuala Lumpur 53100, Malaysia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 06期
关键词：
D O I：
10.1128/AEM.70.6.3769-3771.2004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.
引用
收藏
页码：3769 / 3771
页数：3
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