At least eight types of ubiquitin chain exist, and individual linkages affect distinct cellular processes. The only distinguishing feature of differently linked ubiquitin chains is their structure, as polymers of the same unit are chemically identical. Here, we have crystallized Lys 63-linked and linear ubiquitin dimers, revealing that both adopt equivalent open conformations, forming no contacts between ubiquitin molecules and thereby differing significantly from Lys 48-linked ubiquitin chains. We also examined the specificity of various deubiquitinases (DUBs) and ubiquitin-binding domains (UBDs). All analysed DUBs, except CYLD, cleave linear chains less efficiently compared with other chain types, or not at all. Likewise, UBDs can show chain specificity, and are able to select distinct linkages from a ubiquitin chain mixture. We found that the UBAN (ubiquitin binding in ABIN and NEMO) motif of NEMO (NF-kappa B essential modifier) binds to linear chains exclusively, whereas the NZF (Npl4 zinc finger) domain of TAB2 (TAK1 binding protein 2) is Lys 63 specific. Our results highlight remarkable specificity determinants within the ubiquitin system.
机构:Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Adhikari, A.
;
Xu, M.
论文数: 0引用数: 0
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机构:Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Xu, M.
;
Chen, Z. J.
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h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Ea, CK
;
Deng, L
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h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Deng, L
;
Xia, ZP
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h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Xia, ZP
;
Pineda, G
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h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Pineda, G
;
Chen, ZJJ
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Gyrd-Hansen, Mads
;
Darding, Maurice
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Darding, Maurice
;
Miasari, Maria
论文数: 0引用数: 0
h-index: 0
机构:
La Trobe Univ, Dept Biochem, Bundoora, Vic 3086, AustraliaInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Miasari, Maria
;
Santoro, Massimo M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Turin, Ctr Mol Biotechnol, I-10126 Turin, Italy
Univ Piemonte Orientale, Dept Environm & Life Sci, I-15100 Alessandria, ItalyInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Santoro, Massimo M.
;
Zender, Lars
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USAInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Zender, Lars
;
Xue, Wen
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany
Hannover Med Sch, Dept Gastroenterol Hepatol & Endocrinol, D-30625 Hannover, GermanyInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Xue, Wen
;
Tenev, Tencho
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Tenev, Tencho
;
da Fonseca, Paula C. A.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Sect Struct Biol, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
da Fonseca, Paula C. A.
;
Zvelebil, Marketa
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Zvelebil, Marketa
;
Bujnicki, Janusz M.
论文数: 0引用数: 0
h-index: 0
机构:
Int Inst Mol & Cell Biol Warsaw, PL-02109 Warsaw, Poland
Adam Mickiewicz Univ Poznan, PL-61614 Poznan, PolandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Bujnicki, Janusz M.
;
Lowe, Scott
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USAInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Lowe, Scott
;
Silke, John
论文数: 0引用数: 0
h-index: 0
机构:
La Trobe Univ, Dept Biochem, Bundoora, Vic 3086, AustraliaInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Silke, John
;
Meier, Pascal
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
机构:
Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, IsraelTechnion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
Hershko, A
;
Ciechanover, A
论文数: 0引用数: 0
h-index: 0
机构:Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
机构:Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Adhikari, A.
;
Xu, M.
论文数: 0引用数: 0
h-index: 0
机构:Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Xu, M.
;
Chen, Z. J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Ea, CK
;
Deng, L
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Deng, L
;
Xia, ZP
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Xia, ZP
;
Pineda, G
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
Pineda, G
;
Chen, ZJJ
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USAUniv Texas, SW Med Ctr, Howard Hughes Med Inst, Dept Mol Biol, Dallas, TX 75390 USA
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Gyrd-Hansen, Mads
;
Darding, Maurice
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Darding, Maurice
;
Miasari, Maria
论文数: 0引用数: 0
h-index: 0
机构:
La Trobe Univ, Dept Biochem, Bundoora, Vic 3086, AustraliaInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Miasari, Maria
;
Santoro, Massimo M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Turin, Ctr Mol Biotechnol, I-10126 Turin, Italy
Univ Piemonte Orientale, Dept Environm & Life Sci, I-15100 Alessandria, ItalyInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Santoro, Massimo M.
;
Zender, Lars
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USAInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Zender, Lars
;
Xue, Wen
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany
Hannover Med Sch, Dept Gastroenterol Hepatol & Endocrinol, D-30625 Hannover, GermanyInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Xue, Wen
;
Tenev, Tencho
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Tenev, Tencho
;
da Fonseca, Paula C. A.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Sect Struct Biol, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
da Fonseca, Paula C. A.
;
Zvelebil, Marketa
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Zvelebil, Marketa
;
Bujnicki, Janusz M.
论文数: 0引用数: 0
h-index: 0
机构:
Int Inst Mol & Cell Biol Warsaw, PL-02109 Warsaw, Poland
Adam Mickiewicz Univ Poznan, PL-61614 Poznan, PolandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Bujnicki, Janusz M.
;
Lowe, Scott
论文数: 0引用数: 0
h-index: 0
机构:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USAInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Lowe, Scott
;
Silke, John
论文数: 0引用数: 0
h-index: 0
机构:
La Trobe Univ, Dept Biochem, Bundoora, Vic 3086, AustraliaInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
Silke, John
;
Meier, Pascal
论文数: 0引用数: 0
h-index: 0
机构:
Inst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, EnglandInst Canc Res, Chester Beatty Labs, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
机构:
Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, IsraelTechnion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
Hershko, A
;
Ciechanover, A
论文数: 0引用数: 0
h-index: 0
机构:Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel