STUDY ON GAS CHROMATOGRAPHY- MASS SPECTROMETER FINGERPRINT OF VOLATILE CONSTITUENTS FOR WATER SOLUBLE INGREDIENTS IN PU'ER TEA

The gas chromatography-mass spectrometry (GC-MS) fingerprint method of volatile constituents of the water soluble ingredients in Pu'er tea 7542 (106) was established in paper by comparing it with GC-MS chromatograms data to optimize and explore the volume of the tea extract, the ultrasound extracting time and the temperature and time of oscillation box in static head space (HS). Under the condition of optimization, volatile constituents of the water soluble ingredients in Pu'er tea for different ages and batches 7542 samples were detected by GC-MS. Their GC-MS fingerprint was set up and analyzed their cluster and principal component for reference fingerprint of Pu'er tea 7542 (106) by the traditional Chinese medicine (TCM) chromatographic fingerprint similarity evaluation system (A) 2004 edition software and the SPSS 19.0 software. The result of this experiment: Pu'er tea 7542 was extracted with 30 ml water and 30 min as the extraction by means of ultrasonication to volatile constituents. The extract were detected by HS and GC-MS. The temperature and time of oscillation box in static head space were 120 degrees C and 25min. There were 6 compounds in the volatile components of the water soluble ingredients in Pu'er tea for different ages and batches 7542 samples. These compounds belong to one main characteristic component, the contribution of that accounted for 99.74%. The ten samples for different ages and batches 7542 Pu'er tea had subdivided into four classes.