Accessing the third dimension in localization-based super-resolution microscopy

被引：29
作者：
Hajj, Bassam [1 ,2 ]
El Beheiry, Mohamed [1 ,2 ]
Izeddin, Ignacio [3 ]
Darzacq, Xavier [2 ,3 ]
Dahan, Maxime [1 ,2 ]
机构：
[1] Univ Paris 06, CNRS UMR 168, Inst Curie, Lab Phys Chim Curie, F-75005 Paris, France
[2] Howard Hughes Med Inst, Transcript Imaging Consortium, Ashburn, VA 20147 USA
[3] Ecole Normale Super, CNRS UMR8197, Inst Biol ENS, F-75005 Paris, France
来源：
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2014年 / 16卷 / 31期
关键词：
SINGLE-MOLECULE TRACKING; MULTIFOCAL PLANE MICROSCOPY; FLUORESCENCE MICROSCOPY; MITOCHONDRIAL NUCLEOIDS; 3-DIMENSIONAL TRACKING; DIFFRACTION-LIMIT; PARTICLE-TRACKING; RESOLUTION LIMIT; QUANTUM-DOT; NANOSCOPY;
D O I：
10.1039/c4cp01380h
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Only a few years after its inception, localization-based super-resolution microscopy has become widely employed in biological studies. Yet, it is primarily used in two-dimensional imaging and accessing the organization of cellular structures at the nanoscale in three dimensions (3D) still poses important challenges. Here, we review optical and computational techniques that enable the 3D localization of individual emitters and the reconstruction of 3D super-resolution images. These techniques are grouped into three main categories: PSF engineering, multiple plane imaging and interferometric approaches. We provide an overview of their technical implementation as well as commentary on their applicability. Finally, we discuss future trends in 3D localization-based super-resolution microscopy.
引用
收藏
页码：16340 / 16348
页数：9
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