Use of both direct and indirect 13C tags for probing nitrogen interactions in hairpin ribozyme models by 15N NMR

被引：3

作者：

Shallop, AJ ^{[1
]}

Gaffney, BL ^{[1
]}

Jones, RA ^{[1
]}

机构：

[1] Rutgers State Univ, Dept Chem & Chem Biol, Piscataway, NJ 08854 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2004年 / 23卷 / 1-2期

关键词：

hairpin ribozyme; N-15; NMR; C-13; tag; specific labeling;

D O I：

10.1081/NCN-120027834

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used the synthesis and N-15 NMR study of separate loop A and loop B domains of the hairpin ribozyme to demonstrate that multiple N-15 atoms can be incorporated into an RNA strand and be unambiguously distinguished through a combination of direct and indirect tagging by C-13 atoms. Absence of N-15 chemical shift changes shows that the G8N1 in loop A does not become deprotonated up to pH 8, and that the G21N7 of loop B does not bind to Mg2+.

引用

页码：273 / 280

页数：8

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