Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors

被引：33

作者：

Yamashita, Naoya ^{[1
]}

Usui, Hiroshi ^{[1
]}

Nakamura, Fumio ^{[1
]}

Chen, Sandy ^{[1
]}

Sasaki, Yukio ^{[1
]}

Hida, Tomonobu ^{[1
]}

Suto, Fumikazu ^{[2
]}

Taniguchi, Masahiko ^{[3
]}

Takei, Kohtaro ^{[1
,4
]}

Goshima, Yoshio ^{[1
,4
]}

机构：

[1] Yokohama City Univ, Grad Sch Med, Dept Mol Pharmacol & Neurobiol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Ultrastruct Res, Kodaira, Tokyo 1878502, Japan

[3] Sapporo Med Univ, Sch Med, Dept Cell Sci, Res Inst Frontier Med,Chuo Ku, Sapporo, Hokkaido 0608556, Japan

[4] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

RESPONSE MEDIATOR PROTEIN-1; DIFFERENTIAL PALMITOYLATION; AXOPLASMIC-TRANSPORT; SYNAPTIC PLASTICITY; APICAL DENDRITES; GROWTH CONES; N-CADHERIN; NEURONS; TRAFFICKING; COLLAPSIN;

D O I：

10.1038/ncomms4424

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The dendritic targeting of neurotransmitter receptors is vital for dendritic development and function. However, how such localization is established remains unclear. Here we show that semaphorin 3A (Sema3A) signalling at the axonal growth cone is propagated towards the cell body by retrograde axonal transport and drives AMPA receptor GluA2 to the distal dendrites, which regulates dendritic development. Sema3A enhances glutamate receptor interacting protein 1-dependent localization of GluA2 in dendrites, which is blocked by knockdown of cytoplasmic dynein heavy chain. PlexinA (PlexA), a receptor component for Sema3A, interacts with GluA2 at the immunoglobulin-like Plexin-transcription-factor domain (PlexA-IPT) in somatodendritic regions. Overexpression of PlexA-IPT suppresses dendritic localization of GluA2 and induces aproximal bifurcation phenotype in the apical dendrites of CA1 hippocampal neurons. Thus, we propose a control mechanism by which retrograde Sema3A signalling regulates the glutamate receptor localization through trafficking of cis-interacting PlexA with GluA2 along dendrites.

引用

页数：16

共 57 条

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