Fluorosis is a world wide health problem of both human and animals. It is endemic in those areas where fluoride content of drinking water is very high. The prevalence of fluorosis in animals has been recognized from many parts of India (Swarup and Singh, 1989; Dwivedi et al,. 1997; Sharma et al., 1997, Patra, 2000). In Orissa, the condition has been recognized as endemic problem in human population of some villages of Khurda and Nayagarh district of Orissa (Chaudhury and Naik, 1996; Ray, 2000). However, no report was available on clinical status of animal of in those areas where animals were also taking same drinking water. Therefore, the present study was undertaken to investigate the epidemiological aspects of fluorosis in cattle and to find out the correlation between fluoride level of biological sample and prevalence of fluorosis.