We describe one case of feline leishmaniasis (FL), it is an 5-year-old, spayed female domestic short-haired cat. When she was examinaed, she presented hyporexia, loss weigth, generalized linfadenomegaly, splenomegaly and dermatological examination revealed periocular and tarsal ulcers, dry seborrhoea and abdominal alopecia. Abnormalities in the serum profile were hyperproteinemia and moderate polyclonal gammapathy. The biopsy showed eosinofilic granulomatous dermatiris with a highly dermoepidermical necrosis and ulceration, pattern that could be compatible with leishmaniasis. The real time quantitative PCR (RTQ-PCR) was the test chosen to do the diagnostic. The euthanasia was practiced in our patient, at the owner's request, because the health status deteriorated. No treatment was possible to do and the owners didn't alow to practice any postmortem test to confirm the sistemic implication. In veterinary practice, FL should be included in the diferential diagnosis when compatible simptoms are present, especially in endemic areas of canine leishmaniosis. In addition, the RTQ-PCR provides a useful tool to stablish leishmaniosis diagnosis.