Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria

被引:50
|
作者
Simeonova, DD
Lièvremont, D
Lagarde, F
Muller, DAE
Groudeva, VI
Lett, MC
机构
[1] Univ Strasbourg 1, Lab Dynam Evolut & Express Genomes Microorganisme, CNRS, FRE 2326, F-67083 Strasbourg, France
[2] Univ Strasbourg 1, Lab Chim Analyt & Minerale, CNRS, UMR 7512,Ecole Europeenne Chim Polymeres & Mat, F-67037 Strasbourg, France
[3] Univ Sofia, Dept Gen & Ind Microbiol, Sofia 1421, Bulgaria
关键词
arsenite-oxidation; arsenate-reduction; arsenic speciation; screening method;
D O I
10.1016/j.femsle.2004.06.040
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An efficient, inexpensive microplate colorimetric assay for screening of bacteria which can be used in bioremediation of arsenic was developed. The assay is based on the colorimetric analysis of the precipitates formed upon reaction of silver nitrate with arsenic. The method proved reliable and sensitive for the detection of As[III] oxidizers and As[V] reducers and can be used over a large pH range (5.8-8.4). Seventy-eight bacteria] strains isolated from different environments were tested by this method. It also showed agreement with results obtained by high-performance liquid chromatography coupled with inductively coupled plasma atomic emission spectrometry. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:249 / 253
页数:5
相关论文
共 50 条
  • [1] Biotransformation of adsorbed arsenic on iron minerals by coexisting arsenate-reducing and arsenite-oxidizing bacteria
    Ye, Li
    Wang, Liying
    Jing, Chuanyong
    ENVIRONMENTAL POLLUTION, 2020, 256 (256)
  • [2] Arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in Taiwan
    Chu, Y. -J.
    Hsiao, S. -Y.
    Wei, C. -C.
    Liu, C. -W.
    Liao, C. -M.
    Chang, F. -J.
    Liao, V. H. -C.
    Shen, W. -C.
    ARSENIC IN GEOSPHERE AND HUMAN DISEASES, 2010, : 549 - 550
  • [3] Arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in Taiwan
    Liao, Vivian Hsiu-Chuan
    Chu, Yu-Ju
    Su, Yu-Chen
    Hsiao, Sung-Yun
    Wei, Chia-Cheng
    Liu, Chen-Wuing
    Liao, Chung-Min
    Shen, Wei-Chiang
    Chang, Fi-John
    JOURNAL OF CONTAMINANT HYDROLOGY, 2011, 123 (1-2) : 20 - 29
  • [4] Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia
    Hamamura, Natsuko
    Itai, Takaaki
    Liu, Yitai
    Reysenbach, Anna-Louise
    Damdinsuren, Narantuya
    Inskeep, William P.
    ENVIRONMENTAL MICROBIOLOGY REPORTS, 2014, 6 (05): : 476 - 482
  • [5] Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China
    Fan, H.
    Su, C.
    Wang, Y.
    Yao, J.
    Zhao, K.
    Wang, Y.
    Wang, G.
    JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (02) : 529 - 539
  • [6] A rapid colony screening method for the detection of arsenate-reducing bacteria
    S. M. Mandal
    K. C. Mondal
    S. Dey
    B. R. Pati
    Indian Journal of Microbiology, 2007, 47 (2) : 167 - 169
  • [7] Removal of arsenic from groundwater by arsenite-oxidizing bacteria
    Ike, M.
    Miyazaki, T.
    Yamamoto, N.
    Sei, K.
    Soda, S.
    WATER SCIENCE AND TECHNOLOGY, 2008, 58 (05) : 1095 - 1100
  • [8] Effects of arsenic on the biofilm formations of arsenite-oxidizing bacteria
    Zeng, Xian-Chun
    He, Zhong
    Chen, Xiaoming
    Cao, Qian A. D.
    Li, Hao
    Wang, Yanxin
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2018, 165 : 1 - 10
  • [9] Characterization of arsenite-oxidizing bacteria to decipher their role in arsenic bioremediation
    Biswas, Rimi
    Sarkar, Angana
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2019, 49 (01): : 30 - 37
  • [10] Novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments
    Garcia-Dominguez, Elizabeth
    Mumford, Adam
    Rhine, Elizabeth Danielle
    Paschal, Amber
    Young, Lily Y.
    FEMS MICROBIOLOGY ECOLOGY, 2008, 66 (02) : 401 - 410