Sub-wavelength resolution Fluorescent Correlation Spectroscopy using a Solid Immersion Lens

被引：0

作者：

Serov, A ^{[1
]}

Rao, R ^{[1
]}

Gösch, M ^{[1
]}

Anhut, T ^{[1
]}

Martin, D ^{[1
]}

Brunner, R ^{[1
]}

Rigler, R ^{[1
]}

Lasser, T ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, EPFL, Inst Biomed Imaging Opt & Engn, CH-1015 Lausanne, Switzerland

来源：

ALT'03 INTERNATIONAL CONFERENCE ON ADVANCED LASER TECHNOLOGIES: BIOMEDICAL OPTICS | 2003年 / 5486卷

关键词：

Fluorescent Correlation Spectroscopy; Solid Immersion Lens; single molecule detection; high resolution;

D O I：

10.1117/12.572046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper we present recent spectroscopic studies using a Solid Immersion Lens for Fluorescent Correlation Spectroscopy measurements. We compare the performance of the Solid Immersion Lens confocal microscope built-up in our group to the performance of a conventional confocal microscope used for FCS. The novelty of the new SIL-FCS microscope is a system containing a conventional objective (NA=0.6) combined with a Solid Immersion Lens used for single molecule experiment. Important parameters for single molecule experiments such as collection efficiency and excitation field confinement are investigated for different modes of the SIL objective system.

引用

页码：307 / 314

页数：8

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