Highly active porous scaffolds of collagen and hyaluronic acid prepared by suppression of polyion complex formation

被引：20

作者：

Chen, Shangwu ^{[1
,2
]}

Zhang, Qin ^{[1
,2
]}

Nakamoto, Tomoko ^{[1
]}

Kawazoe, Naoki ^{[1
]}

Chen, Guoping ^{[1
,2
]}

机构：

[1] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton, Tissue Regenerat Mat Unit, Tsukuba, Ibaraki 3050044, Japan

[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Dept Mat Sci & Engn, Tsukuba, Ibaraki 3058571, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2014年 / 2卷 / 34期

关键词：

INFRARED CORRELATION SPECTROSCOPY; GLYCOSAMINOGLYCAN SCAFFOLDS; SECONDARY STRUCTURE; MECHANICAL-PROPERTIES; CROSS-LINKING; PORE-SIZE; CONTRACTION; PROLIFERATION; PROTEINS; DESIGN;

D O I：

10.1039/c4tb00780h

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Collagen-hyaluronic acid scaffolds with high bioactivity, good mechanical properties and homogeneous pore structures are desirable for their applications in tissue engineering. However, under aqueous conditions collagen and hyaluronic acid form polyion complexes (PIC), which results in heterogeneous structures and poor mechanical properties of the scaffolds. In this study, we used low molecular weight salts to suppress PIC formation in collagen-hyaluronic acid suspensions during scaffold preparation. The suppression of PIC formation was studied by using turbidimetry, viscosity measurement and infrared analysis. The effects of suppression of PIC formation on the morphology and mechanical properties of the scaffolds were examined using scanning electron microscopy and compression tests. PIC formation was found to be dependent on the ionic strength of the suspension. The secondary structure of collagen was partially altered by its strong electrostatic interactions with hyaluronic acid. The suppression of PIC formation resulted in collagen-hyaluronic acid scaffolds with homogeneous pore structures and remarkably enhanced mechanical properties. Collagen-hyaluronic acid scaffolds prepared under suppression of PIC formation promoted proliferation of fibroblasts and upregulated the expression of genes encoding EGF, VEGF and IGF-1. Using low molecular weight salts to suppress PIC formation could aid in the design of collagen-glycosaminoglycan scaffolds for tissue engineering.

引用

页码：5612 / 5619

页数：8

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