In this study, the effects of ethanol and unleaded gasoline with 1, 4 Dioxan blends on multi-cylinder SI engine were investigated. The test fuels were prepared using 99.9% pure ethanol and gasoline with 1,4 Dioxan blend, in the ratio of E 50+5 1,4 Dioxan, E 60+101,4 Dioxan, the rest gasoline. In this work, the performance and emission tests were conducted in a multi-cylinder petrol engine. The experimental results reveal the increase in brake thermal efficiency for the blends when compared to that of sole fuel. In this investigation, the emission tests are made with the help of AVL Di Gas analyzer, in which CO, CO2, HC, NOx are appreciably reduced and O-2 is increased for all the blends when compared to sole fuel.