Localization of 5-HT4 receptor mRNA in rat brain by in situ hybridization histochemistry

被引：99
作者：
Vilaro, MT
Cortes, R
Gerald, C
Branchek, TA
Palacios, JM
Mengod, G
机构：
[1] CSIC,DEPT NEUROCHEM,INST INVEST BIOMED,ES-08034 BARCELONA,SPAIN
[2] SYNAPT PHARMACEUT CORP,PARAMUS,NJ 07652
[3] LABS ALMIRALI,RES INST,BARCELONA 08024,SPAIN
来源：
MOLECULAR BRAIN RESEARCH | 1996年 / 43卷 / 1-2期
关键词：
5-HT4; receptor; splice variant; I-125]SB 207710; basal ganglia; pre-synaptic receptor;
D O I：
10.1016/S0169-328X(96)00248-3
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Oligonucleotide probes that recognize two cloned splice variants (5-HT4S and 5-HT4L) of 5-HT4 receptors were used to study by in situ hybridization the localization in rat brain of mRNA encoding these receptors. A probe common to both variants reveals high levels of transcripts in olfactory tubercle, some components of the basal ganglia (caudate putamen, ventral striatum), medial habenula and hippocampal formation. Similar patterns of distribution are obtained with probes that recognize each splice variant individually, suggesting that no dramatic differences exist in their respective regional distribution. Comparison of mRNA distribution with receptor distribution as visualized with [I-125]SB 207710 indicates that 5-HT4 receptors are localized both somatodendritically in e.g. caudate putamen and on axon terminals in e.g. substantia nigra and globus pallidus.
引用
收藏
页码：356 / 360
页数：5
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