Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1

被引：12

作者：

McClernon, DR ^{[1
]}

Vavro, C ^{[1
]}

Clair, MS ^{[1
]}

机构：

[1] GkaxoSmithKline, Dept Clin Virol, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 06期

关键词：

D O I：

10.1128/JCM.02187-05

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.

引用

页码：2280 / 2282

页数：3

共 7 条

[1] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].

BOOM, R ;

SOL, CJA ;

SALIMANS, MMM ;

JANSEN, CL ;

WERTHEIMVANDILLEN, PME ;

VANDERNOORDAA, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503

[2] Multicenter evaluation of the NucliSens EasyQ HIV-1 v1.1 assay for the quantitative detection of HIV-1 RNA in plasma [J].

de Mendoza, C ;

Koppelman, M ;

Montès, B ;

Ferre, V ;

Soriano, V ;

Cuypers, H ;

Segondy, M ;

Oosterlaken, T .

JOURNAL OF VIROLOGICAL METHODS, 2005, 127 (01) :54-59

[3] Characteristics and applications of nucleic acid sequence-based amplification (NASBA) [J].

Deiman, B ;

van Aarle, P ;

Sillekens, P .

MOLECULAR BIOTECHNOLOGY, 2002, 20 (02) :163-179

[4] Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma [J].

Murphy, DG ;

Côté, L ;

Fauvel, M ;

René, P ;

Vincelette, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4034-4041

[5] Impact of clade diversity on HIV-1 virulence, antiretroviral drug sensitivity and drug resistance [J].

Spira, S ;

Wainberg, MA ;

Loemba, H ;

Turner, D ;

Brenner, BG .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 (02) :229-240

[6] Evaluation of the NucliSens EasyQ assay in HIV-1-infected individuals in South Africa [J].

Stevens, W ;

Wiggill, T ;

Horsfield, P ;

Coetzee, L ;

Scott, LE .

JOURNAL OF VIROLOGICAL METHODS, 2005, 124 (1-2) :105-110

[7] Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay [J].

Yao, J ;

Liu, Z ;

Ko, LS ;

Pan, G ;

Jiang, Y .

JOURNAL OF VIROLOGICAL METHODS, 2005, 129 (01) :40-46

← 1 →