Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers

被引：558
作者：
Yu, Xin
Munge, Bernard
Patel, Vyomesh
Jensen, Gary
Bhirde, Ashwin
Gong, Joseph D.
Kim, Sang N.
Gillespie, John
Gutkind, J. Silvio
Papadimitrakopoulos, Fotios
Rusling, James F.
机构：
[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
[2] Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA
[3] Salve Regina Univ, Dept Chem, Newport, RI 02840 USA
[4] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA
[5] NCI, Pathogenet Unit, Ctr Adv Technol, Pathol Lab,Ctr Canc Res, Bethesda, MD 20892 USA
[6] NCI, Urol Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA
[7] Natl Inst Dent & Craniofacial Res, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 34期
关键词：
PROSTATE-SPECIFIC ANTIGEN; ELECTROCHEMICAL DETECTION; ANALYTICAL PERFORMANCE; SYSTEMS BIOLOGY; IMMUNOASSAY; FUNCTIONALIZATION; IMMUNOSENSORS; PEROXIDASE; CHALLENGES; PROTEOMICS;
D O I：
10.1021/ja062117e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We describe herein the combination of electrochemical immunosensors using single-wall carbon nanotube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sensitive detection of a cancer biomarker in serum and tissue lysates. Greatly amplified sensitivity was attained by using bioconjugates featuring horseradish peroxidase (HRP) labels and secondary antibodies (Ab(2)) linked to carbon nanotubes (CNT) at high HRP/Ab(2) ratio. This approach provided a detection limit of 4 pg mL(-1) (100 amol mL(-1)), for prostate specific antigen (PSA) in 10 mu L of undiluted calf serum, a mass detection limit of 40 fg. Accurate detection of PSA in human serum samples was demonstrated by comparison to standard ELISA assays. PSA was quantitatively measured in prostate tissue samples for which PSA could not be differentiated by the gold standard immunohistochemical staining method. These easily fabricated SWNT immunosensors show excellent promise for clinical screening of cancer biomarkers and point-of-care diagnostics.
引用
收藏
页码：11199 / 11205
页数：7
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