Well-Defined Macromolecules Using Horseradish Peroxidase as a RAFT Initiase

被引：67

作者：

Danielson, Alex P. ^{[1
]}

Bailey-Van Kuren, Dylan ^{[1
]}

Lucius, Melissa E. ^{[1
]}

Makaroff, Katherine ^{[1
]}

Williams, Cameron ^{[1
]}

Page, Richard C. ^{[1
]}

Berberich, Jason A. ^{[2
]}

Konkolewicz, Dominik ^{[1
]}

机构：

[1] Miami Univ, Dept Chem & Biochem, 651 E High St, Oxford, OH 45056 USA

[2] Miami Univ, Dept Chem Paper & Biomed Engn, Miami, FL USA

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2016年 / 37卷 / 04期

关键词：

enzyme catalysis; heme proteins; polymerization; protein modifications; raft polymerization; FREE-RADICAL POLYMERIZATION; ENZYME-MEDIATED INITIATION; ACRYLAMIDE POLYMERIZATION; METHYL-METHACRYLATE; CHAIN TRANSFER; GRAFTING-FROM; PROTEIN; TEMPERATURE; POLYMERS;

D O I：

10.1002/marc.201500633

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Enzymatic catalysis and control over macromolecular architectures from reversible addition-fragmentation chain transfer polymerization (RAFT) are combined to give a new method of making polymers. Horseradish peroxidase (HRP) is used to catalytically generate radicals using hydrogen peroxide and acetylacetone as a mediator. RAFT is used to control the polymer structure. HRP catalyzed RAFT polymerization gives acrylate and acrylamide polymers with relatively narrow molecular weight distributions. The polymerization is rapid, typically exceeding 90% monomer conversion in 30 min. Complex macromolecular architectures including a block copolymer and a protein-polymer conjugate are synthesized using HRP to catalytically initiate RAFT polymerization.

引用

页码：362 / 367

页数：6

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