Effects and mechanism of ultrasound pretreatment on rapeseed protein enzymolysis

被引：52

作者：

Jin, Jian ^{[1
]}

Ma, Haile ^{[1
,2
]}

Wang, Weiwei ^{[1
]}

Luo, Min ^{[1
]}

Wang, Bei ^{[1
]}

Qu, Wenjuan ^{[1
]}

He, Ronghai ^{[1
,2
]}

Owusu, John ^{[1
,3
]}

Li, Yunliang ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China

[2] Jiangsu Prov Key Lab Phys Proc Agr Prod, Zhenjiang 212013, Jiangsu, Peoples R China

[3] Koforidua Polytech, Sch Appl Sci & Technol, Dept Hospitality, POB 981, Koforidua, Ghana

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2016年 / 96卷 / 04期

基金：

中国国家自然科学基金;

关键词：

rapeseed protein; ultrasound; kinetics; thermodynamics; circular dichroism; scanning electron microscopy; ACE-INHIBITORY PEPTIDES; WHEAT-GERM PROTEIN; FUNCTIONAL-PROPERTIES; PHYSICAL-PROPERTIES; KINETICS; HYDROLYSIS; OPTIMIZATION; SPECTROSCOPY; GLOBULIN; COLLAGEN;

D O I：

10.1002/jsfa.7198

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

BACKGROUNDThe disadvantages which stem from the use of traditional enzymolysis of protein has necessitated the need to employ sweeping frequency and pulsed ultrasound (SFPU) in the pretreatment of rapeseed protein prior to proteolysis in order to bring about improvement in enzymolysis efficiency. Further, in order to determine the mechanism of ultrasound-accelerated enzymolysis of RP, the effects of SFPU on the kinetics, thermodynamics, molecular conformation and microstructure of RP were investigated. RESULTSKinetic studies showed that SFPU pretreatment on RP improved enzymolysis by decreasing the apparent constant K-M significantly (P < 0.05) by 32.8% and reducing the thermodynamic parameters E-a, H and S by 16.6%, 17.7% and 9.2% respectively. Fluorescence spectra revealed that SFPU pretreatment induced molecular unfolding, causing more hydrophobic groups and regions inside the molecules to be exposed to the outside. Circular dichroism analysis indicated that SFPU pretreatment decreased the -helix content by 16.1% and increased the random coil content by 3.6%. In addition, scanning electron microscopy showed that SFPU pretreatment increased the specific surface area of RP. CONCLUSIONUltrasound pretreatment is an efficient method in RP proteolysis to produce peptides through its impact on the molecular conformation and microstructure of proteins. (c) 2015 Society of Chemical Industry

引用

页码：1159 / 1166

页数：8

共 44 条

[21] Effects of ultrasound on the structure and physical properties of black bean protein isolates [J].

Jiang, Lianzhou ;

Wang, Jing ;

Li, Yang ;

Wang, Zhongjiang ;

Liang, Jing ;

Wang, Rui ;

Chen, Yong ;

Ma, Wenjun ;

Qi, Baokun ;

Zhang, Min .

FOOD RESEARCH INTERNATIONAL, 2014, 62 :595-601

[22] Effects of multi-frequency power ultrasound on the enzymolysis and structural characteristics of corn gluten meal [J].

Jin, Jian ;

Ma, Haile ;

Wang, Kai ;

Yagoub, Abu El-Gasim A. ;

Owusu, John ;

Qu, Wenjuan ;

He, Ronghai ;

Zhou, Cunshan ;

Ye, Xiaofei .

ULTRASONICS SONOCHEMISTRY, 2015, 24 :55-64

[23] Ultrasonic inactivation of Bacillus α-amylase.: I.: effect of gas content and emitting face of probe [J].

Kadkhodaee, Rassoul ;

Povey, Malcolm J. W. .

ULTRASONICS SONOCHEMISTRY, 2008, 15 (02) :133-142

[24] A standard method to calibrate sonochemical efficiency of an individual reaction system [J].

Koda, S ;

Kimura, T ;

Kondo, T ;

Mitome, H .

ULTRASONICS SONOCHEMISTRY, 2003, 10 (03) :149-156

[25] VIBRATIONAL SPECTROSCOPY AND CONFORMATION OF PEPTIDES, POLYPEPTIDES, AND PROTEINS [J].

KRIMM, S ;

BANDEKAR, J .

ADVANCES IN PROTEIN CHEMISTRY, 1986, 38 :181-364

[26] Identification and Inhibitory Properties of Multifunctional Peptides from Pea Protein Hydrolysate [J].

Li, Huan ;

Aluko, Rotimi F. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (21) :11471-11476

[27] Effect of energy-gathered ultrasound on Alcalase [J].

Ma, Haile ;

Huang, Liurong ;

Jia, Junqiang ;

He, Ronghai ;

Luo, Lin ;

Zhu, Wenxue .

ULTRASONICS SONOCHEMISTRY, 2011, 18 (01) :419-424

[28] Structural changes induced in proteins by therapeutic ultrasounds [J].

Marchioni, C. ;

Riccardi, E. ;

Spinelli, S. ;

Dell'Unto, F. ;

Grimaldi, P. ;

Bedini, A. ;

Giliberti, C. ;

Giuliani, L. ;

Palomba, R. ;

Castellano, A. Congiu .

ULTRASONICS, 2009, 49 (6-7) :569-576

[29] Enzymolysis reaction kinetics and thermodynamics of defatted wheat germ protein with ultrasonic pretreatment [J].

Qu, Wenjuan ;

Ma, Haile ;

Liu, Bin ;

He, Ronghai ;

Pan, Zhongli ;

Abano, Ernest Ekow .

ULTRASONICS SONOCHEMISTRY, 2013, 20 (06) :1408-1413

[30] Enzymolysis kinetics and activities of ACE inhibitory peptides from wheat germ protein prepared with SFP ultrasound-assisted processing [J].

Qu, Wenjuan ;

Ma, Haile ;

Jia, Junqiang ;

He, Ronghai ;

Luo, Lin ;

Pan, Zhongli .

ULTRASONICS SONOCHEMISTRY, 2012, 19 (05) :1021-1026

← 1 2 3 4 5 →