Neuroprotective effect of methanol extract of Phellodendri Cortex against 1-methyl-4-phenylpyridinium (MPP+)-induced apoptosis in PC-12 cells

被引：34
作者：
Jung, Hyo Won
Jin, Guang-Zhen [2 ]
Kim, Sung Yun [2 ]
Kim, Yeong Shik [3 ]
Park, Yong-Ki [1 ,2 ]
机构：
[1] Dongguk Univ, Coll Oriental Med, Dept Herbol, Oriental Med R&D Ctr, Gyeongju 780714, South Korea
[2] Dongguk Univ, Oriental Med Res Inst, Gyeongju 780714, South Korea
[3] Seoul Natl Univ, Coll Pharm, Inst Nat Prod Res, Seoul 151747, South Korea
来源：
CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 09期
关键词：
Apoptosis; Neuroprotection; PC-12; cells; Phellodendri Cortex; 1-Methyl-4-phenylpyridinium; CELLULAR IMMUNE-RESPONSE; NERVE GROWTH-FACTOR; PC12; CELLS; DEATH; NEUROTOXICITY; BCL-2; ANTIOXIDANT; PROTECTS; AMURENSE; NEURONS;
D O I：
10.1016/j.cellbi.2009.06.006
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Phellodendri Cortex (PC) is a traditional herbal medicine, widely used in Korea and China. The effects of the methanol extract of Phellodendri Cortex (PC extract) on 1-methyl-4-phenylpyridinium (MPP+)-induced neuronal apoptosis in PC-12 cells have been investigated. MPP+-induced apoptosis in PC-12 cells was accompanied by an increased bax/bcl-2 ratio, release of cytochrome c to the cytosol and activation of caspase-3. PC extract inhibited the downregulation of bcl-2 and the upregulation of bax, as well as the release of mitochondrial cytochrome c into the cytosol. In addition, PC extract attenuated caspase-3 activation and cleavage of poly (ADP-ribose) polymerase (PARP). These results suggest that the PC extract has protective effects against MPP+-induced neuronal apoptosis in PC-12 cells. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：957 / 963
页数：7
相关论文
共 35 条
[1]  
Cadet JL, 2000, SYNAPSE, V35, P228
[2]   Curcumin protects PC12 cells against 1-methyl-4-phenylpyridinium ion-induced apoptosis by Bcl-2-mitochondria-ROS-iNOS pathway [J].
Chen, J. ;
Tang, X. Q. ;
Zhi, J. L. ;
Cui, Y. ;
Yu, H. M. ;
Tang, E. H. ;
Sun, S. N. ;
Feng, J. Q. ;
Chen, P. X. .
APOPTOSIS, 2006, 11 (06) :943-953
[3]   Vitamin E blocks early events induced by 1-methyl-4-phenylpyridinium (MPP+) in cerebellar granule cells [J].
González-Polo, RA ;
Soler, G ;
Alvarez, A ;
Fabregat, I ;
Fuentes, JM .
JOURNAL OF NEUROCHEMISTRY, 2003, 84 (02) :305-315
[4]   Angiotensin II attenuates chemical hypoxia-induced caspase-3 activation in primary cortical neuronal cultures [J].
Grammatopoulos, TN ;
Morris, K ;
Bachar, C ;
Moore, S ;
Andres, R ;
Weyhenmeyer, JA .
BRAIN RESEARCH BULLETIN, 2004, 62 (04) :297-303
[5]   ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].
GREENE, LA ;
TISCHLER, AS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2424-2428
[6]   BCL-2 family members and the mitochondria in apoptosis [J].
Gross, A ;
McDonnell, JM ;
Korsmeyer, SJ .
GENES & DEVELOPMENT, 1999, 13 (15) :1899-1911
[7]   Protocatechuic acid suppresses MPP+-induced mitochondrial dysfunction and apoptotic cell death in PC12 cells [J].
Guan, S. ;
Jiang, B. ;
Bao, Y. M. ;
An, L. J. .
FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (10) :1659-1666
[8]   Methylpyridinium (MPP+)- and nerve growth factor-induced changes in pro- and anti-apoptotic signaling pathways in SH-SY5Y neuroblastoma cells [J].
Halvorsen, EM ;
Dennis, J ;
Keeney, PM ;
Sturgill, TW ;
Tuttle, JB ;
Bennett, JP .
BRAIN RESEARCH, 2002, 952 (01) :98-110
[9]  
HOSHI A, 1976, GANN, V67, P321
[10]   Bel-2 protects against Aβ25-35-induced oxidative PC12 cell death by potentiation of antioxidant capacity [J].
Jang, JH ;
Surh, YJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 320 (03) :880-886
1234