Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein

被引：1
作者：
Brun, F
Schaefer, DG
Laloue, M
Gonneau, M
机构：
[1] INRA, Biol Cellulaire Lab, F-78026 Versailles, France
[2] Univ Lausanne, Inst Ecol, Lab Phytogenet Cellulaire, CH-1015 Lausanne, Switzerland
来源：
PLANT SCIENCE | 2004年 / 167卷 / 03期
关键词：
allergen; gene disruption; intracellular PR proteins; Physcomitrella patens;
D O I：
10.1016/j.plantsci.2004.04.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
UBP34. a soluble 34 kDa protein identified in the moss Physcomitrella patens by photoaffinity labeling with an urea-type cytokinin agonist, belonging to the plant intracellular pathogenesis related (IPR) protein family. This class of proteins is ubiquitous in the plant kingdom but their functions are still unknown. Using a reverse genetic approach we generated knockout mutants at the UBP34 locus by homologous recombination. Knockout plants grown in various conditions do not present any visible phenotypic alteration. However, biochemical and molecular analysis of the knockout transformants reveal the specific photoaffinity labeling of another similar protein. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：471 / 479
页数：9
相关论文
共 18 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   ANALYSIS OF GAMETOPHYTIC DEVELOPMENT IN THE MOSS, PHYSCOMITRELLA-PATENS, USING AUXIN AND CYTOKININ RESISTANT MUTANTS [J].
ASHTON, NW ;
GRIMSLEY, NH ;
COVE, DJ .
PLANTA, 1979, 144 (05) :427-435
[3]   Crystal structures of two homologous pathogenesis-related proteins from yellow lupine [J].
Biesiadka, J ;
Bujacz, G ;
Sikorski, MM ;
Jaskolski, M .
JOURNAL OF MOLECULAR BIOLOGY, 2002, 319 (05) :1223-1234
[4]   Molecular characterization of a cytokinin-inducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the Bet v 1 allergen family [J].
Carpin, S ;
Laffer, S ;
Schoentgen, F ;
Valenta, R ;
Chénieux, JC ;
Rideau, M ;
Hamdi, S .
PLANT MOLECULAR BIOLOGY, 1998, 36 (05) :791-798
[5]  
Dellaporta S. L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]
[6]   Purification and cDNA cloning of cytokinin-specific binding protein from mung bean (Vigna radiata) [J].
Fujimoto, Y ;
Nagata, R ;
Fukasawa, H ;
Yano, K ;
Azuma, M ;
Iida, A ;
Sugimoto, S ;
Shudo, K ;
Hashimoto, Y .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 258 (02) :794-802
[7]   Photoaffinity labelling with the cytokinin agonist azido-CPPU of a 34 kDa peptide of the intracellular pathogenesis-related protein family in the moss Physcomitrella patens [J].
Gonneau, M ;
Pagant, S ;
Brun, F ;
Laloue, M .
PLANT MOLECULAR BIOLOGY, 2001, 46 (05) :539-548
[8]   Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions [J].
HoffmannSommergruber, K ;
VanekKrebitz, M ;
Radauer, C ;
Wen, J ;
Ferreira, F ;
Scheiner, O ;
Breiteneder, H .
GENE, 1997, 197 (1-2) :91-100
[9]   A specific member of the Cab multigene family can be efficiently targeted and disrupted in the moss Physcomitrella patens [J].
Hofmann, AH ;
Codón, AC ;
Ivascu, C ;
Russo, VEA ;
Knight, C ;
Cove, D ;
Schaefer, DG ;
Chakhparonian, M ;
Zryd, JP .
MOLECULAR AND GENERAL GENETICS, 1999, 261 (01) :92-99
[10]  
HULL R, 2002, MATTHEWS PLANT VIROL, P796
12