Intracellular dynamics of topoisomerase I inhibitor, CPT-11, by slit-scanning confocal Raman microscopy

被引：33

作者：

Harada, Yoshinori ^{[1
]}

Dai, Ping ^{[1
]}

Yamaoka, Yoshihisa ^{[1
]}

Ogawa, Mitsugu ^{[1
]}

Tanaka, Hideo ^{[1
]}

Nosaka, Kazuto ^{[2
]}

Akaji, Kenichi ^{[2
]}

Takamatsu, Tetsuro ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Pathol & Cell Regulat, Kamigyo Ku, Kyoto 6028566, Japan

[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Chem, Kita Ku, Kyoto 6038334, Japan

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2009年 / 132卷 / 01期

关键词：

Slit-scanning Raman microscopy; Molecular imaging; Non-labeling method; Anticancer-drug; CANCER-CELLS; SPECTROSCOPY; PROTEIN; TISSUE; DRUG; ACCUMULATION; RESISTANCE; SN-38;

D O I：

10.1007/s00418-009-0594-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Most molecular imaging technologies require exogenous probes and may have some influence on the intracellular dynamics of target molecules. In contrast, Raman scattering light measurement can identify biomolecules in their innate state without application of staining methods. Our aim was to analyze intracellular dynamics of topoisomerase I inhibitor, CPT-11, by using slit-scanning confocal Raman microscopy, which can take Raman images with high temporal and spatial resolution. We could acquire images of the intracellular distribution of CPT-11 and its metabolite SN-38 within several minutes without use of any exogenous tags. Change of subcellular drug localization after treatment could be assessed by Raman imaging. We also showed intracellular conversion from CPT-11 to SN-38 using Raman spectra. The study shows the feasibility of using slit-scanning confocal Raman microscopy for the non-labeling evaluation of the intracellular dynamics of CPT-11 with high temporal and spatial resolution. We conclude that Raman spectromicroscopic imaging is useful for pharmacokinetic studies of anticancer drugs in living cells.

引用

页码：39 / 46

页数：8

共 26 条

[1] ABCG2 mediates differential resistance to SN-38 (7-ethyl-10-hydroxycamptothecin) and homocamptothecins [J].