Identification of human TGF-β1 signal (leader) sequence polymorphisms by PCR-RFLP

被引：47
作者：
Wood, NAP
Thomson, SC
Smith, RM
Bidwell, JL
机构：
[1] Univ Bristol, Dept Pathol & Microbiol, Bristol BS6 6JU, Avon, England
[2] Univ Bristol, Southmead Hosp, Acad Renal Unit, Bristol BS10 5NB, Avon, England
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 234卷 / 1-2期
关键词：
TGF-beta; polymorphism; RFLP; electrophoresis;
D O I：
10.1016/S0022-1759(99)00127-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Links between disease susceptibility and genetically determined variation in human cytokine expression have recently been described. This has led to a demand for simple methods of identifying cytokine gene polymorphisms of potential clinical relevance. Here, we describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying two human transforming growth factor beta 1 (TGF-beta 1) signal (leader) sequence polymorphisms, T869C (Leu10Pro) and G915C (Arg25Pro). This permits simple and robust identification of TGF-beta 1 leader sequence genotypes and demonstrates the physical linkage in cis between T869C (Leu10Pro) and G915C (Arg25Pro). The method does not require previously genotyped standards. The efficacy of enzyme digestion is internally controlled by the presence of conserved restriction sites. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：117 / 122
页数：6
相关论文
共 19 条
[11]   TGF-β1 DNA polymorphisms, protein levels, and blood pressure [J].
Li, BG ;
Khanna, A ;
Sharma, V ;
Singh, T ;
Suthanthiran, M ;
August, P .
HYPERTENSION, 1999, 33 (01) :271-275
[12]   Rapid genotyping of transforming growth factor β1 gene polymorphisms in a UK Caucasoid control population using the polymerase chain reaction and sequence-specific primers [J].
Lympany, PA ;
Avila, JJ ;
Mullighan, C ;
Marshall, S ;
Welsh, KI ;
du Bois, RM .
TISSUE ANTIGENS, 1998, 52 (06) :573-578
[13]   A transformed view of cyclosporine [J].
Nabel, GJ .
NATURE, 1999, 397 (6719) :471-472
[14]   Genotyping for polymorphisms in interferon-γ, interleukin-10, transforming growth factor-β1 and tumour necrosis factor-α genes:: a technical report [J].
Perrey, C ;
Pravica, V ;
Sinnott, PJ ;
Hutchinson, IV .
TRANSPLANT IMMUNOLOGY, 1998, 6 (03) :193-197
[15]  
Pociot F, 1998, J AM SOC NEPHROL, V9, P2302
[16]   Molecular genetics of coronary artery disease: measuring the phenotype [J].
Samani, NJ .
CLINICAL SCIENCE, 1998, 95 (06) :645-646
[17]   Transforming growth factor-β gene polymorphisms and coronary artery disease [J].
Syrris, P ;
Carter, ND ;
Metcalfe, JC ;
Kemp, PR ;
Grainger, DJ ;
Kaski, JC ;
Crossman, DC ;
Francis, SE ;
Gunn, J ;
Jeffrey, S ;
Heathcote, K .
CLINICAL SCIENCE, 1998, 95 (06) :659-667
[18]   A common polymorphism of the transforming growth factor-β1 gene and coronary artery disease [J].
Wang, XL ;
Sim, AS ;
Wilcken, DEL .
CLINICAL SCIENCE, 1998, 95 (06) :745-746
[19]   Association of a polymorphism of the transforming growth factor-β1 gene with genetic susceptibility to osteoporosis in postmenopausal Japanese women [J].
Yamada, Y ;
Miyauchi, A ;
Goto, J ;
Takagi, Y ;
Okuizumi, H ;
Kanematsu, M ;
Hase, M ;
Takai, H ;
Harada, A ;
Ikeda, K .
JOURNAL OF BONE AND MINERAL RESEARCH, 1998, 13 (10) :1569-1576
12