In vitro culture of rainbow trout, Oncorhynchus mykiss, gonads during sex differentiation and gonadogenesis

被引：0

作者：

Guillevic, Mathieu ^{[1
]}

Mourot, Brigitte ^{[1
]}

Guiguen, Yann ^{[1
]}

Fostier, Alexis ^{[1
]}

机构：

[1] INRA, UR 1037 SCRIBE, IFR 140, F-35000 Rennes, France

来源：

CYBIUM | 2008年 / 32卷 / 02期

关键词：

Embryonic gonad; In vitro culture; Rainbow trout; Sexual differentiation; Estradiol;

D O I：

暂无

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The histological features, steroidogenic activities (aromatase, I I P-hydroxylase) and gene expressions (cyp19A1, cyp11b1, amh, ddx4, gcl, fst) of trout embryonic gonads have been compared before and after 7 days of in vitro culture at 12 degrees C in three different media. MCDB 105 gave the best results and was used to analyse the effect of 50 nM 17 beta-estradiol on testicular cyp11b1 expression. A significant six-fold decrease has been observed for cyp11b1, while amh, ddx4 and gcl did not change.

引用

页码：107 / 108

页数：2