Proteomic response of oat leaves to long-term salinity stress

被引:33
作者
Bai, Jianhui [1 ,2 ]
Qin, Yan [1 ]
Liu, Jinghui [2 ]
Wang, Yuqing [1 ]
Sa, Rula [2 ]
Zhang, Na [2 ]
Jia, Ruizong [3 ]
机构
[1] Chinese Acad Agr Sci, Inst Grassland Res, Hohhot 010010, Peoples R China
[2] Inner Mongolia Agr Univ, Sci Innovat Team Oats, Hohhot 010019, Peoples R China
[3] Hawaii Agr Res Ctr, Kunia, HI 96759 USA
基金
中国国家自然科学基金;
关键词
Salinity stress; Oat; Proteomics; Antioxidant enzymes; Photosynthesis; VACUOLAR H+-ATPASE; RIBOSOMAL-PROTEIN; SALT TOLERANCE; ANTIOXIDATIVE ENZYMES; GENOTYPE IMPACT; DROUGHT STRESS; RICE; ARABIDOPSIS; ISOFORMS; GROWTH;
D O I
10.1007/s11356-016-8092-0
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Salinity adversely affects plant growth and production. Oat is a moderately salt-tolerant crop and can contribute to improving saline soil. The physiological and molecular responses of the oat plant to long-term salinity were studied. After a 16-day salt treatment (150 mmol L-1NaCl in Hoagland's solution), photosynthetic rate, maximum photosystem II photochemical efficiency, and actual efficiency of photosystem II decreased. The activities of superoxide dismutase, peroxidase, and catalase significantly increased. We also investigated the protein profiles of oat leaves in response to salinity and detected 30 reproducible protein spots by two-dimensional gel electrophoresis that were differentially abundant. Specifically, one protein was up-regulated and 29 proteins were down-regulated compared with the control. These 29 proteins were identified using MALDI-TOF mass spectrometry, and 19 corresponding genes were further investigated by quantitative real-time PCR. These proteins were involved in four types of biological processes: photosynthesis, carbohydrate metabolism and energy, protein biosynthesis, and folding and detoxification. This study indicates that the lower levels of Calvin cycle-related proteins, 50S ribosomal protein L10 and adenosine-triphosphate regulation-related proteins, and the high levels of antioxidant enzymes play important roles in the response of oat to long-term salinity stress.
引用
收藏
页码:3387 / 3399
页数:13
相关论文
共 53 条
[1]  
Abdel-Aal E, 2004, HULLESS OATS, P223
[2]   Proteome analysis of soybean hypocotyl and root under salt stress [J].
Aghaei, K. ;
Ehsanpour, A. A. ;
Shah, A. H. ;
Komatsu, S. .
AMINO ACIDS, 2009, 36 (01) :91-98
[3]   Salt tolerance and activity of antioxidative enzymes of transgenic finger millet overexpressing a vacuolar H+-pyrophosphatase gene (SbVPPase) from Sorghum bicolor [J].
Anjaneyulu, Ediga ;
Reddy, Palle Surender ;
Sunita, Merla Srilakshmi ;
Kishor, Polavarapu B. Kavi ;
Meriga, Balaji .
JOURNAL OF PLANT PHYSIOLOGY, 2014, 171 (10) :789-798
[4]   A DIGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism [J].
Bohler, Sacha ;
Bagard, Matthieu ;
Oufir, Mouhssin ;
Planchon, Sebastien ;
Hoffmann, Lucien ;
Jolivet, Yves ;
Hausman, Jean-Francois ;
Dizengremel, Pierre ;
Renaut, Jenny .
PROTEOMICS, 2007, 7 (10) :1584-1599
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   14-3-3 protein is a regulator of the mitochondrial and chloroplast ATP synthase [J].
Bunney, TD ;
van Walraven, HS ;
de Boer, AH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (07) :4249-4254
[7]   Root protein metabolism in association with improved root growth and drought tolerance by elevated carbon dioxide in creeping bentgrass [J].
Burgess, Patrick ;
Huang, Bingru .
FIELD CROPS RESEARCH, 2014, 165 :80-91
[8]   Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice [J].
Campbell, Malachy T. ;
Knecht, Avi C. ;
Berger, Bettina ;
Brien, Chris J. ;
Wang, Dong ;
Walia, Harkamal .
PLANT PHYSIOLOGY, 2015, 168 (04) :1476-U1697
[9]   The effect of mutating arginine-469 on the substrate binding and refolding activities of 70-kDa heat shock cognate protein [J].
Chang, TC ;
Hsiao, CD ;
Wu, SJ ;
Wang, C .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 386 (01) :30-36
[10]   Weak microwave can alleviate water deficit induced by osmotic stress in wheat seedlings [J].
Chen, Yi-Ping ;
Jia, Jing-Fen ;
Han, Xiao-Ling .
PLANTA, 2009, 229 (02) :291-298