Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

被引：32

作者：

Adhikari, Deepak ^{[1
]}

Diril, M. Kasim ^{[2
]}

Busayavalasa, Kiran ^{[1
]}

Risal, Sanjiv ^{[1
]}

Nakagawa, Shoma ^{[3
]}

Lindkvist, Rebecca ^{[1
]}

Shen, Yan ^{[1
]}

Coppola, Vincenzo ^{[4
]}

Tessarollo, Lino ^{[4
]}

Kudo, Nobuaki R. ^{[3
]}

Kaldis, Philipp ^{[2
,5
]}

Liu, Kui ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, S-40530 Gothenburg, Sweden

[2] ASTAR, Inst Mol & Cell Biol, Singapore 138673, Singapore

[3] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Inst Reprod & Dev Biol, Dept Surg & Canc, London W12 0NN, England

[4] NCI, Mouse Canc Genet Program, Frederick, MD 21702 USA

[5] Natl Univ Singapore, Dept Biochem, Singapore 117599, Singapore

来源：

JOURNAL OF CELL BIOLOGY | 2014年 / 206卷 / 07期

基金：

英国医学研究理事会; 瑞典研究理事会;

关键词：

PROTEIN PHOSPHATASE 2A; CELL-CYCLE REGULATION; GREATWALL KINASE; MEIOTIC MATURATION; PHOSPHORYLATION; MITOSIS; PROGRESSION; CHECKPOINT; OOCYTES; ARPP19;

D O I：

10.1083/jcb.201406033

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

引用

页码：843 / 853

页数：11

共 59 条

[1] Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals [J].