Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

被引：46

作者：

Asare-Okai, P. N. ^{[1
]}

Agustin, E. ^{[1
]}

Fabris, D. ^{[1
]}

Royzen, M. ^{[1
]}

机构：

[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 58期

关键词：

CLICK CHEMISTRY; LIVING CELLS; ENZYMATIC INCORPORATION; DNA; TRANSCRIPTION; POLYMERASE; FUNCTIONALIZATION; CYCLOADDITION; CONJUGATION; MOLECULES;

D O I：

10.1039/c4cc02435d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

引用

收藏

页码：7844 / 7847

页数：4

相关论文

共 37 条

[11] Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry [J].

Ishizuka, Takumi ;

Kimoto, Michiko ;

Sato, Akira ;

Hirao, Ichiro .

CHEMICAL COMMUNICATIONS, 2012, 48 (88) :10835-10837

[12] Exploring RNA transcription and turnover in vivo by using click chemistry [J].

Jao, Cindy Y. ;

Salic, Adrian .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (41) :15779-15784

[13] Nonpolar Nucleoside Mimics as Active Substrates for Human Thymidine Kinases [J].

Jarchow-Choy, Sarah K. ;

Sjuvarsson, Elena ;

Sintim, Herman O. ;

Eriksson, Staffan ;

Kool, Eric T. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (15) :5488-5494

[14] Synthesis and Evaluation of a Series of 1,2,4,5-Tetrazines for Bioorthogonal Conjugation [J].

Karver, Mark R. ;

Weissleder, Ralph ;

Hilderbrand, Scott A. .

BIOCONJUGATE CHEMISTRY, 2011, 22 (11) :2263-2270

[15] Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry [J].

Kumar, Ravindra ;

El-Sagheer, Afaf ;

Tumpane, John ;

Lincoln, Per ;

Wilhelmsson, L. Marcus ;

Brown, Tom .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (21) :6859-6864

[16] Diels-Alder Cycloaddition for Fluorophore Targeting to Specific Proteins inside Living Cells [J].

Liu, Daniel S. ;

Tangpeerachaikul, Anupong ;

Selvaraj, Ramajeyam ;

Taylor, Michael T. ;

Fox, Joseph M. ;

Ting, Alice Y. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (02) :792-795

[17] Strain-Promoted "Click" Chemistry for Terminal Labeling of DNA [J].

Marks, Isaac S. ;

Kang, Jun Sung ;

Jones, Brady T. ;

Landmark, Kevin J. ;

Cleland, Andrew J. ;

Taton, T. Andrew .

BIOCONJUGATE CHEMISTRY, 2011, 22 (07) :1259-1263

[18] ABORTIVE PRODUCTS AS INITIATING NUCLEOTIDES DURING TRANSCRIPTION BY T7 RNA-POLYMERASE [J].

MORONEY, SE ;

PICCIRILLI, JA .

BIOCHEMISTRY, 1991, 30 (42) :10343-10349

[19] Visualization of Dynamics of Single Endogenous mRNA Labeled in Live Mouse [J].

Park, Hye Yoon ;

Lim, Hyungsik ;

Yoon, Young J. ;

Follenzi, Antonia ;

Nwokafor, Chiso ;

Lopez-Jones, Melissa ;

Meng, Xiuhua ;

Singer, Robert H. .

SCIENCE, 2014, 343 (6169) :422-424

[20] T7 RNA polymerase produces 5′ end heterogeneity during in vitro transcription from certain templates [J].

Pleiss, JA ;

Derrick, ML ;

Uhlenbeck, OC .

RNA, 1998, 4 (10) :1313-1317

← 1 2 3 4 →