Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

被引：46

作者：

Asare-Okai, P. N. ^{[1
]}

Agustin, E. ^{[1
]}

Fabris, D. ^{[1
]}

Royzen, M. ^{[1
]}

机构：

[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 58期

关键词：

CLICK CHEMISTRY; LIVING CELLS; ENZYMATIC INCORPORATION; DNA; TRANSCRIPTION; POLYMERASE; FUNCTIONALIZATION; CYCLOADDITION; CONJUGATION; MOLECULES;

D O I：

10.1039/c4cc02435d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

引用

收藏

页码：7844 / 7847

页数：4

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