β-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris

被引：6

作者：

Ayra-Pardo, C ^{[1
]}

Montejo-Sierra, IL ^{[1
]}

Vázquez-Padrón, RI ^{[1
]}

García-Martínez, C ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Plant Div, Havana, Cuba

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1999.00610.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and beta-D-glucuronidase (beta-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene dosage, beta-GUS was functionally expressed and easily detected in all studied clones. The results obtained demonstrate the feasibility of using this bacterial enzyme as a reporter in Pichia pastoris.

引用

页码：278 / 283

页数：6

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