Extracellular secretion of feruloyl esterase derived from Lactobacillus crispatus in Escherichia coli and its application for ferulic acid production

被引：44

作者：

Xu, Zhenshang ^{[1
,2
]}

Wang, Ting ^{[1
,2
]}

Zhang, Susu ^{[3
]}

机构：

[1] Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Shandong, Peoples R China

[2] Qilu Univ Technol, Shandong Acad Sci, Dept Bioengn, Shandong Prov Key Lab Microbial Engn, Jinan 250353, Shandong, Peoples R China

[3] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 288卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Feruloyl esterase; Lactobacillus crispatus; Secretory production; Escherichia coli; Ferulic acid; CINNAMOYL ESTERASES; CATABOLIC PATHWAY; KEY ENZYME; OPTIMIZATION; VANILLIN; PURIFICATION; EXPRESSION; BACTERIA;

D O I：

10.1016/j.biortech.2019.121526

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A feruloyl esterase producing strain was isolated and identified as Lactobacillus crispatus 5524. Its putative feruloyl esterase was heterogeneously expressed in Escherichia toll BL21 (DE3). Interestingly, the feruloyl esterase (FaeLcr) could be secreted into the culture medium with a relative high purity of 201.7 mg/L. FaeLcr was purified from the cell-free culture supernatant and appeared as a single protein band with the molecular mass of 28 kDa by SDS-PAGE. The optimal temperature and pH were determined as 65 degrees C and 7.0, and it showed prominent thermo-stability and alkali-stability. Furthermore, the purified FarLcr could release a maximal amount of 199 mu g ferulic acid from 0.2 g de-starched wheat bran. Meanwhile, when cultured this recombinant E. coli. strain in medium supplemented with 2 g de-starched wheat bran, 1.86 mg ferulic acid was also detected. These results suggested that the recombinant strain has a great potential application in feruloyl esterase and ferulic acid production.

引用

页数：8

共 38 条

[1] Administration of Lactobacillus fermentum CRL1446 increases intestinal feruloyl esterase activity in mice [J].