Convergent Evolution of Endosymbiont Differentiation in Dalbergioid and Inverted Repeat-Lacking Clade Legumes Mediated by Nodule-Specific Cysteine-Rich Peptides

被引：103

作者：

Czernic, Pierre ^{[1
]}

Gully, Djamel ^{[2
]}

Cartieaux, Fabienne ^{[2
]}

Moulin, Lionel ^{[2
]}

Guefrachi, Ibtissem ^{[3
]}

Patrel, Delphine ^{[2
]}

Pierre, Olivier ^{[3
]}

Fardoux, Joel ^{[2
]}

Chaintreuil, Clemence ^{[2
]}

Phuong Nguyen ^{[2
]}

Gressent, Frederic ^{[2
]}

Da Silva, Corinne ^{[4
]}

Poulain, Julie ^{[4
]}

Wincker, Patrick ^{[4
]}

Rofidal, Valerie ^{[5
]}

Hem, Sonia ^{[5
]}

Barriere, Quentin ^{[3
]}

Arrighi, Jean-Franois ^{[2
]}

Mergaert, Peter ^{[3
]}

Giraud, Eric ^{[2
]}

机构：

[1] Univ Montpellier, F-34095 Montpellier 5, France

[2] Univ Montpellier, Lab Symbioses Trop & Mediterraneennes, Ctr Cooperat Int Rech Agron Dev, SupAgro,Inst Natl Rech Agron,Inst Rech Dev, F-34398 Montpellier 5, France

[3] Univ Paris 11, Commissariat Energie Atom, Ctr Natl Rech Sci, Inst Integrat Biol Cell,Unite Mixte Rech 9198, F-91198 Gif Sur Yvette, France

[4] Genoscope, Inst Genom, Commissariat Energie Atom, Direct Sci Vivant, F-91000 Evry, France

[5] INRA, Lab Proteom Fonct, Unite Rech 1199, F-34060 Montpellier, France

来源：

PLANT PHYSIOLOGY | 2015年 / 169卷 / 02期

关键词：

MEDICAGO-TRUNCATULA; PHOTOSYNTHETIC BRADYRHIZOBIA; SYMBIOSIS; AESCHYNOMENE; RHIZOBIUM; BACTERIA; GENES; MODEL; PLANT; NODULATION;

D O I：

10.1104/pp.15.00584

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Nutritional symbiotic interactions require the housing of large numbers of microbial symbionts, which produce essential compounds for the growth of the host. In the legume-rhizobium nitrogen-fixing symbiosis, thousands of rhizobium microsymbionts, called bacteroids, are confined intracellularly within highly specialized symbiotic host cells. In Inverted Repeat-Lacking Clade (IRLC) legumes such as Medicago spp., the bacteroids are kept under control by an arsenal of nodule-specific cysteine-rich (NCR) peptides, which induce the bacteria in an irreversible, strongly elongated, and polyploid state. Here, we show that in Aeschynomene spp. legumes belonging to the more ancient Dalbergioid lineage, bacteroids are elongated or spherical depending on the Aeschynomene spp. and that these bacteroids are terminally differentiated and polyploid, similar to bacteroids in IRLC legumes. Transcriptome, in situ hybridization, and proteome analyses demonstrated that the symbiotic cells in the Aeschynomene spp. nodules produce a large diversity of NCR-like peptides, which are transported to the bacteroids. Blocking NCR transport by RNA interference-mediated inactivation of the secretory pathway inhibits bacteroid differentiation. Together, our results support the view that bacteroid differentiation in the Dalbergioid clade, which likely evolved independently from the bacteroid differentiation in the IRLC clade, is based on very similar mechanisms used by IRLC legumes.

引用

页码：1254 / 1265

页数：12

共 37 条

[1] Genomic organization and evolutionary insights on GRP and NCR genes, two large nodule-specific gene families in Medicago truncatula [J].