PCR Detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus Ticks in Mongolia

被引：23

作者：

Masuzawa, Toshiyuki ^{[1
]}

Masuda, Shou ^{[1
]}

Fukui, Takashi ^{[1
]}

Okamoto, Yoshihiro ^{[1
]}

Bataa, Jantsandoo ^{[5
]}

Oikawa, Yosaburo ^{[2
]}

Ishiguro, Fubito ^{[3
]}

Takada, Nobuhiro ^{[4
]}

机构：

[1] CIS, Fac Pharm, Lab Microbiol & Immunol, Choshi 2880025, Japan

[2] Kanazawa Med Univ, Lab Med Zool, Kanazawa, Ishikawa 9200293, Japan

[3] Fukui Prefectural Inst Publ Hlth, Fukui 9108551, Japan

[4] Univ Fukui, Fac Med Sci, Sr Fellow Off, Fukui 9101193, Japan

[5] Mongolian Natl Invest Ctr Infect Dis, Ulaanbaatar, Mongolia

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2014年 / 67卷 / 01期

基金：

日本学术振兴会;

关键词：

SENSU-LATO; LYME-BORRELIOSIS; WESTERN SIBERIA; AGENT; EHRLICHIA; RODENTS; PREVALENCE; INFECTION; PROVINCE; GARINII;

D O I：

10.7883/yoken.67.47

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).

引用

页码：47 / 49

页数：3

共 26 条

[1] Human granulocytic ehrlichiosis in Europe
Blanco, JR
Oteo, JA
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2002, 8 (12) : 763 - 772
[2] Prevalence of Anaplasma phagocytophila and Borrelia burgdorferi in Ixodes persulcatus ticks from northeastern China
Cao, WC
Zhao, QM
Zhang, PH
Yang, H
Wu, XM
Wen, BH
Zhang, XT
Habbema, JDF
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 68 (05) : 547 - 550
[3] Natural Anaplasma phagocytophilum infection of ticks and rodents from a forest area of Jilin Province, China
Cao, Wu-Chun
Zhan, Lin
He, Jing
Foley, Janet E.
De Vlas, Sake J.
Wu, Xiao-Ming
Yang, Hong
Richardus, Jan. H.
Habbema, J. Dik F.
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2006, 75 (04) : 664 - 668
[4] IDENTIFICATION OF A GRANULOCYTOTROPIC EHRLICHIA SPECIES AS THE ETIOLOGIC AGENT OF HUMAN-DISEASE
CHEN, SM
DUMLER, JS
BAKKEN, JS
WALKER, DH
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) : 589 - 595
[5] EHRLICHIAL DISEASES OF HUMANS - EMERGING TICK-BORNE INFECTIONS
DUMLER, JS
BAKKEN, JS
[J]. CLINICAL INFECTIOUS DISEASES, 1995, 20 (05) : 1102 - 1110
[6] Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales:: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila
Dumler, JS
Barbet, AF
Bekker, CPJ
Dasch, GA
Palmer, GH
Ray, SC
Rikihisa, Y
Rurangirwa, FR
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 : 2145 - 2165
[7] Heterogeneity of the ospA gene structure from isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and Mongolia
Fomenko, N. V.
Stronin, O. V.
Khasnatinov, M. N.
Danchinova, G. A.
Bataa, J.
Gol'tsova, N. A.
[J]. MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, 2009, 24 (04) : 183 - 188
[8] Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis:: Life in the wilds
Gern, L.
[J]. PARASITE, 2008, 15 (03) : 244 - 247
[9] Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp in wild deer and ticks on two major islands in Japan
Kawahara, M
Rikihisa, Y
Lin, Q
Isogai, E
Tahara, K
Itagaki, A
Hiramitsu, Y
Tajima, T
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (02) : 1102 - 1109
[10] Tik-borne-borne rickettsial pathogens in ticks and small mammals in Korea
Kim, Chul-Min
Yi, Ying-Hua
Yu, Do-Hyeon
Lee, Mi-Jin
Cho, Mae-Rim
Desai, Atul R.
Shringi, Smriti
Klein, Terry A.
Kim, Heung-Chul
Song, Jin-Won
Back, Luck-Ju
Chong, Sung-Tae
O'Guinn, Monica L.
Lee, John S.
Lee, In-Yong
Park, Jin-Ho
Foley, Janet
Chae, Joon-Seok
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (09) : 5766 - 5776

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