Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry

被引：32

作者：

Su, Juan ^{[1
]}

Zhang, Chuan ^{[1
]}

Zhang, Wei ^{[1
]}

Shen, Yun-heng ^{[1
]}

Li, Hui-liang ^{[1
]}

Liu, Run-hui ^{[1
]}

Zhang, Xi ^{[1
]}

Hu, Xiao-jia ^{[2
]}

Zhang, Wei-dong ^{[1
,2
]}

机构：

[1] Second Mil Med Univ, Dept Nat Med Chem, Sch Pharm, Shanghai 200433, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 11期

关键词：

Coumarins; Zushima; Thymelaeaceae; HPLC-DAD-ESI-MS; Identification; Quantification; DAPHNE-GIRALDII;

D O I：

10.1016/j.chroma.2008.06.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method, high-performance liquid chromatography coupled with diode array and electrospray tandem mass spectrometry (HPLC-DAD-ESI-MS), was developed to qualitatively identify and quantitatively determine the 10 major active coumarins of Zushima. The analysis was performed by using a ZORBAX SB-C18 analytical Column (250 mm x 4.6 mm ID, 5 mu m) at gradient elution of 0.5% aqueous formic acid and acetonitrile with diode array detection (325 nm). The method was validated for linearity, precision, accuracy, limit of detection and quantification. The proposed method was Successfully applied for the qualitative and quantitative analysis Of 10 coumarins in five different species of Zushima which had great variation on the contents of investigated coumarins. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：2111 / 2117

页数：7

共 25 条

[1] CHEN MJ, 2002, CHIN TRAD PAT MED, V24, P905
[2] DANG QP, 2006, J TRADIT CHIN MED, V27, P734
[3] Analysis of nicotinoid insecticides residues in honey by solid matrix partition clean-up and liquid chromatography-electrospray mass spectrometry
Fidente, P
Seccia, S
Vanni, F
Morrica, P
[J]. JOURNAL OF CHROMATOGRAPHY A, 2005, 1094 (1-2) : 175 - 178
[4] Jiangsu New Medical College, 1977, DICT CHIN HERB MED, P1739
[5] Rapid quantification and characterization of soyasaponins by high-performance liquid chromatography coupled with electrospray mass spectrometry
Jin, MC
Yang, YW
Su, BG
Ren, QL
[J]. JOURNAL OF CHROMATOGRAPHY A, 2006, 1108 (01) : 31 - 37
[6] Li S.H., 2002, J CHIN MAT MED, V27, P401
[7] A new dicoumarinoid glycoside from Daphne giraldii
Li, SH
Wu, LJ
Gao, HY
Chen, YH
Li, Y
[J]. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH, 2005, 7 (06) : 839 - 842
[8] LIN L, 1988, J CHEM RES, V12, P396
[9] Liu G, 1995, Zhongguo Zhong Yao Za Zhi, V20, P738
[10] EDGEWORTHIN, A NEW BIS-COUMARIN FROM EDGEWORTHIA-GARDNERI
MAJUMDER, PL
SENGUPTA, GC
DINDA, BN
CHATTERJEE, A
[J]. PHYTOCHEMISTRY, 1974, 13 (09) : 1929 - 1931

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