A novel function for a ubiquitous plant enzyme pectin methylesterase:: The enhancer of RNA silencing

被引：43

作者：

Dorokhov, Yuri L.

Frolova, Olga Y.

Skurat, Eugene V.

Ivanov, Peter A.

Gasanova, Tatjana V.

Sheveleva, Anna A.

Ravin, Nikolay V.

Makinen, Kristiina M.

Klimyuk, Victor I.

Skryabin, Konstantin G.

Gleba, Yuri Y.

Atabekov, Joseph G.

机构：

[1] Moscow MV Lomonosov State Univ, Dept Virol, Moscow 119992, Russia

[2] Moscow MV Lomonosov State Univ, AN Belozersky Inst PhysicoChem Biol, Moscow 119992, Russia

[3] Russian Acad Sci, Ctr Bioengn, Moscow, Russia

[4] Univ Helsinki, Dept Appl Biol, FIN-00014 Helsinki, Finland

[5] Icon Genet GmbH, Biozentrum, Halle, Germany

来源：

FEBS LETTERS | 2006年 / 580卷 / 16期

基金：

俄罗斯基础研究基金会;

关键词：

pectin methylesterase; tobacco mosaic virus; RNA silencing;

D O I：

10.1016/j.febslet.2006.06.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Co-agroinjection of Nicotiana henthamiana leaves with the pectin methylesterase (proPME) gene and the TMV:GFP vector resulted in a stimulation of virus-induced RNA silencing (inhibition of GFP production, virus RNA degradation, stimulation of siRNAs production). Conversely, coexpression of TMV:GFP with either antisense PME construct or with enzymatically inactive proPME restored synthesis of viral RNA. Furthermore, expression of proPME enhanced the GFP transgene-induced gene silencing accompanied by relocation of the DCL1 protein from nucleus to the cytoplasm and activation of siRNAs and miRNAs production. It was hypothesized that DCL1 relocated to the cytoplasm may use as substrates both miRNA precursor and viral RNA. The capacity for enhancing the RNA silencing is a novel function for the polyfunctional PME. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3872 / 3878

页数：7

共 26 条

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