Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium

被引：56
|
作者：
Song, M
Zhu, N
Olcese, R
Barila, B
Toro, L
Stefani, E
机构：
[1] Univ Calif Los Angeles, Dept Anesthesiol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, Dept Physiol, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Sch Med, Inst Brain Res, Los Angeles, CA 90095 USA
来源：
FEBS LETTERS | 1999年 / 460卷 / 03期
关键词：
K channel; protein expression; myometrium; hormone; mRNA;
D O I：
10.1016/S0014-5793(99)01394-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Large conductance voltage-dependent and Ca2+-modulated K+ channels play a crucial role in myometrium contractility, Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na+/K+-ATPase protein and RNA levels do not significantly; change at different stages of pregnancy, The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：427 / 432
页数：6
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