Whole genome sequencing and metabolomics analyses reveal the biosynthesis of nerol in a multi-stress-tolerant Meyerozyma guilliermondii GXDK6

被引：20

作者：

Mo, Xueyan ^{[1
]}

Cai, Xinghua ^{[1
]}

Hui, Qinyan ^{[1
]}

Sun, Huijie ^{[1
]}

Yu, Ran ^{[1
]}

Bu, Ru ^{[1
]}

Yan, Bing ^{[2
]}

Ou, Qian ^{[1
]}

Li, Quanwen ^{[1
]}

He, Sheng ^{[3
]}

Jiang, Chengjian ^{[1
,2
]}

机构：

[1] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangxi Res Ctr Microbial & Enzyme Engn Technol, Coll Life Sci & Technol, Nanning 530004, Peoples R China

[2] Guangxi Acad Sci, Guangxi Mangrove Res Ctr, Guangxi Key Lab Mangrove Conservat & Utilizat, Beihai 536000, Peoples R China

[3] Maternal & Child Hlth Hosp Guangxi Zhuang Autonom, Guangxi Birth Defects Prevent & Control Inst, Nanning 530033, Peoples R China

来源：

MICROBIAL CELL FACTORIES | 2021年 / 20卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Meyerozyma guilliermondii; Whole genome sequencing; Metabolomics technology; Aroma-producing mechanism; Biosynthesis; PICHIA-ANOMALA; IDENTIFICATION; PROBIOTICS; FERMENTATION; DEGRADATION; ENZYME;

D O I：

10.1186/s12934-020-01490-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Nerol (C10H18O), an acyclic monoterpene, naturally presents in plant essential oils, and is used widely in food, cosmetics and pharmaceuticals as the valuable fragrance. Meanwhile, chemical synthesis is the only strategy for large-scale production of nerol, and the disadvantages of chemical synthesis greatly limit the production and its application. These defects drive the interests of researchers shift to the production of nerol by eco-friendly methods known as biosynthesis methods. However, the main technical bottleneck restricting the biosynthesis of nerol is the lacking of corresponding natural aroma-producing microorganisms. Results: In this study, a novel multi-stress-tolerant probiotics Meyerozymaguilliermondii GXDK6 with aroma-producing properties was identified by whole genome sequencing and metabolomics technology. GXDK6 showed a broad pH tolerance in the range of 2.5-10.0. The species also showed salt tolerance with up to 12% NaCl and up to 18% of KCl or MgCl2. GXDK6 exhibited heavy-metal Mn2+ tolerance of up to 5494 ppm. GXDK6 could also ferment with a total of 21 kinds of single organic matter as the carbon source, and produce abundant aromatic metabolites. Results from the gas chromatography-mass spectrometry indicated the production of 8-14 types of aromatic metabolites (isopentanol, nerol, geraniol, phenylethanol, isobutanol, etc.) when GXDK6 was fermented up to 72 h with glucose, sucrose, fructose, or xylose as the single carbon source. Among them, nerol was found to be a novel aromatic metabolite from GXDK6 fermentation, and its biosynthesis mechanism had also been further revealed. Conclusion: A novel aroma-producing M. guilliermondii GXDK6 was identified successfully by whole genome sequencing and metabolomics technology. GXDK6 showed high multi-stress-tolerant properties with acid-base, salty, and heavy-metal environments. The aroma-producing mechanism of nerol in GXDK6 had also been revealed. These findings indicated the aroma-producing M. guilliermondii GXDK6 with multi-stress-tolerant properties has great potential value in the fermentation industry.

引用

页数：11