Pulicaria crispa mitigates gastric ulcer induced by ethanol in rats: role of treatment and auto healing

被引：23

作者：

Fahmi, Abdelgawad A. ^{[1
]}

Abdur-Rahman, Mariam ^{[1
]}

Naser, Asmaa F. Aboul ^{[2
]}

Hamed, Manal A. ^{[2
]}

Abd-Alla, Howaida I. ^{[3
]}

Nasr, Mohamed I. ^{[4
]}

机构：

[1] Cairo Univ, Fac Sci, Dept Chem, Cairo, Egypt

[2] Natl Res Ctr, Dept Therapeut Chem, El Buhouth St, Giza, Egypt

[3] Natl Res Ctr, Chem Nat Cpds Dept, Giza, Egypt

[4] Cairo Specialized Hosp, Clin Pathol Lab, Cairo, Egypt

来源：

BIOMARKERS | 2019年 / 24卷 / 03期

关键词：

Gastric ulcer; ethanol; Pulicaria crispa; enzymes; antioxidant; inflammatory mediators; histopathology; ESSENTIAL OILS; ANTIOXIDANT; TOXICITY; EXTRACTS; LESIONS; INJURY; L;

D O I：

10.1080/1354750X.2018.1556340

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Context: Stomach ulcers are the common gastrointestinal disorders worldwide. Objective: This study aimed to investigate the therapeutic impact of Pulicaria crispa aerial parts ethanol extract against gastric ulcer in rats. Materials and methods: Ulcer was induced by one oral dose of ethanol (0.5 ml/100g body weight) on 24 hours empty stomach, then the plant extract (500 mg/kg b.wt.) was orally administered daily for one week. Ranitidine (100 mg/kg b.wt.); as a reference drug was evaluated. Stomach acidity and volume, as well as lesion counts were measured. Levels of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were estimated. Assay of different marker enzymes; succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glucose-6-phosphatase (G-6-Pase), acid phosphatase (AP) and 5 '-nucleotidase (5 ' NT) were determined. Interlukin-10 (IL-10), intracellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor alpha (TNF-alpha) were also determined. Stomach histopathological assessment was detected. Results: Gastric ulcer showed drastic changes in oxidative stress, cell organelles and inflammatory markers. These biomarkers served as good tools to identify the presence of gastric ulcer. Treatment with P. crispa recorded amelioration in most parameters exceeding the auto healing effect. Conclusion: Healing potency of P. crispa is possibly related to its content of glycosides, coumarins, flavonoids, tannins, sterols and triterpenes.

引用

页码：286 / 294

页数：9