Quantitative analysis of expression levels of bax, bcl-2, and survivin in cancer cells during cisplatin treatment

被引:1
作者
Ikeguchi, M [1 ]
Nakamura, S [1 ]
Kaibara, N [1 ]
机构
[1] Tottori Univ, Fac Med, Dept Surg 1, Yonago, Tottori 6838504, Japan
关键词
apoptosis; bax; bcl-2; survivin; cisplatin; real-time RT-PCR;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cisplatin (CDDP) exerts significant activity against a wide variety of human malignancies. However, sensitivity to CDDP differs among cancer cells. CDDP induces apoptosis in cancer cells. In the present study, to evaluate good markers of chemo-sensitivity or chemo-resistance of cancer cells, the correlation between occurrence of apoptosis and the changes in expression levels of messenger RNA (mRNA) of three genes (bax, bcl-2, and survivin) in cancer cell lines during CDDP treatment were investigated. Cells (MKN-45, LoVo, and PANC-1) were incubated with CDDP (10 mug/ml). The percentage of cells in sub-G1 fraction was measured by flow cytometry. The changes in expression levels of three genes (bax, bcl-2, and survivin) during CDDP treatment were evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The percentage of cells in sub-G1 fraction increased after a shorter incubation with CDDP in LoVo cells and also increased between 12 and 24 h CDDP treatment in MKN-45 cells. On the other hand, even with a 24 h incubation with CDDP, the percentage of cells in sub-G1 fraction did not change in PANC-1 cells. The expression level of bax mRNA significantly increased after 24 h treatment with CDDP in MKN-45 cells and it significantly increased after 12 h treatment with CDDP in LoVo cells. Also, in LoVo dwells, the expression level of bcl-2 mRNA decreased after-24 h treatment with CDDP. On the other hand, during CDDP treatment, the expression levels of bcl-2 and survivin mRNA significantly increased in PANC-1 cells. These findings indicate that-during chemotherapy, changes in expression levels of bax, bcl-2, and survivin may provide information about chemo-sensitivity or the chemo-resistance of tumors.
引用
收藏
页码:1121 / 1126
页数:6
相关论文
共 26 条
[11]  
IKEGUCHI M, 1995, ONCOL REP, V2, P1027
[12]   EVALUATION OF A METHOD FOR QUANTITATIVE IMMUNOHISTOCHEMICAL ANALYSIS OF CISPLATIN-DNA ADDUCTS IN TISSUES FROM NUDE-MICE [J].
JOHNSSON, A ;
OLSSON, C ;
ANDERSON, H ;
CAVALLINSTAHL, E .
CYTOMETRY, 1994, 17 (02) :142-150
[13]  
Kato J, 2001, INT J CANCER, V95, P92
[14]   Bax induction activates apoptotic cascade via mitochondrial cytochrome c release and Bax overexpression enhances apoptosis induced by chemotherapeutic agents in DLD-1 colon cancer cells [J].
Kobayashi, T ;
Sawa, H ;
Morikawa, J ;
Zhang, W ;
Shiku, H .
JAPANESE JOURNAL OF CANCER RESEARCH, 2000, 91 (12) :1264-1268
[15]   Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood [J].
Kruse, N ;
Pette, M ;
Toyka, K ;
Rieckmann, P .
JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 210 (02) :195-203
[16]   Control of apoptosis and mitotic spindle checkpoint by survivin [J].
Li, FZ ;
Ambrosini, G ;
Chu, EY ;
Plescia, J ;
Tognin, S ;
Marchisio, PC ;
Altieri, DC .
NATURE, 1998, 396 (6711) :580-584
[17]   Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade [J].
Li, P ;
Nijhawan, D ;
Budihardjo, I ;
Srinivasula, SM ;
Ahmad, M ;
Alnemri, ES ;
Wang, XD .
CELL, 1997, 91 (04) :479-489
[18]  
Mahotka C, 1999, CANCER RES, V59, P6097
[19]   Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel [J].
Mitas, M ;
Mikhitarian, K ;
Walters, C ;
Baron, PL ;
Elliott, BM ;
Brothers, TE ;
Robison, JG ;
Metcalf, JS ;
Palesch, YY ;
Zhang, Z ;
Gillanders, WE ;
Cole, DJ .
INTERNATIONAL JOURNAL OF CANCER, 2001, 93 (02) :162-171
[20]   Apoptosis by death factor [J].
Nagata, S .
CELL, 1997, 88 (03) :355-365