Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro

被引：11
作者：
Pandey, Sony [1 ]
Hickey, Dawn U. [1 ]
Drum, Marti [1 ]
Millis, Darryl L. [1 ]
Cekanova, Maria [1 ,2 ]
机构：
[1] Univ Tennessee, Coll Vet Med, Dept Small Anim Clin Siences, POB 1071, Knoxville, TN 37996 USA
[2] Univ Tennessee, UT ORNL Grad Sch Genome Sci & Technol, Knoxville, TN 37996 USA
来源：
BMC VETERINARY RESEARCH | 2019年 / 15卷 / 1期
关键词：
Canine; Cell viability; Platelet-rich plasma; Osteoarthritis; Regenerative medicine; Multipotent mesenchymal stromal cells; Bone marrow; ANIMAL SERUM; STEM-CELLS; PRP; OSTEOARTHRITIS; EXPANSION; THERAPY; TIME;
D O I：
10.1186/s12917-019-2010-x
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
BackgroundReported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay.ResultsPositive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs.ConclusionPRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.
引用
收藏
页数：8
相关论文
共 37 条
[1]   Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma [J].
Chen, Wei-Hong ;
Liu, Hen-Yu ;
Lo, Wen-Cheng ;
Wu, Shinn-Chih ;
Chi, Chau-Hwa ;
Chang, Hsueh-Yuan ;
Hsiao, Shih-Hsiang ;
Wu, Chih-Hsiung ;
Chiu, Wen-Ta ;
Chen, Bao-Ji ;
Deng, Win-Ping .
BIOMATERIALS, 2009, 30 (29) :5523-5533
[2]   Effect of platelet-rich plasma (PRIP) concentration on the viability and proliferation of alveolar bone cells:: an in vitro study [J].
Choi, BH ;
Zhu, SJ ;
Kim, BY ;
Huh, JY ;
Lee, SH ;
Jung, JH .
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2005, 34 (04) :420-424
[3]   Effect of platelet-rich plasma on bone regeneration in autogenous bone graft [J].
Choi, BH ;
Im, CJ ;
Huh, JY ;
Suh, JJ ;
Lee, SH .
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2004, 33 (01) :56-59
[4]  
CLEMMONS RM, 1983, THROMB HAEMOSTASIS, V50, P838
[5]   Mesenchymal stem cell therapy for knee osteoarthritis. Preliminary report of four patients [J].
Davatchi, Fereydoun ;
Abdollahi, Bahar Sadeghi ;
Mohyeddin, Mandana ;
Shahram, Farhad ;
Nikbin, Behrooz .
INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES, 2011, 14 (02) :211-215
[6]   No effects of PRP on ultrasonographic tendon structure and neovascularisation in chronic midportion Achilles tendinopathy [J].
de Vos, R. J. ;
Weir, A. ;
Tol, J. L. ;
Verhaar, J. A. N. ;
Weinans, H. ;
van Schie, H. T. M. .
BRITISH JOURNAL OF SPORTS MEDICINE, 2011, 45 (05) :387-392
[7]   Platelet-rich plasma therapy - future or trend? [J].
Dhillon, Robinder S. ;
Schwarz, Edward M. ;
Maloney, Michael D. .
ARTHRITIS RESEARCH & THERAPY, 2012, 14 (04)
[8]   Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applications [J].
Doucet, C ;
Ernou, I ;
Zhang, YZ ;
Llense, JR ;
Begot, L ;
Holy, X ;
Lataillade, JJ .
JOURNAL OF CELLULAR PHYSIOLOGY, 2005, 205 (02) :228-236
[9]   Platelet-rich plasma: why intra-articular? A systematic review of preclinical studies and clinical evidence on PRP for joint degeneration [J].
Filardo, G. ;
Kon, E. ;
Roffi, A. ;
Di Matteo, B. ;
Merli, M. L. ;
Marcacci, M. .
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 2015, 23 (09) :2459-2474
[10]   Platelet-Rich Plasma From Basic Science to Clinical Applications [J].
Foster, Timothy E. ;
Puskas, Brian L. ;
Mandelbaum, Bert R. ;
Gerhardt, Michael B. ;
Rodeo, Scott A. .
AMERICAN JOURNAL OF SPORTS MEDICINE, 2009, 37 (11) :2259-2272
1234