Rapid method for the determination of the substitution pattern of O-methylated 1,4-glucans by high-pH anion-exchange chromatography with pulsed amperometric detection

被引：23
作者：
Heinrich, J [1 ]
Mischnick, P [1 ]
机构：
[1] UNIV HAMBURG,INST ORGAN CHEM,D-20146 HAMBURG,GERMANY
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 749卷 / 1-2期
关键词：
carbohydrates; glucans;
D O I：
10.1016/0021-9673(96)00468-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid method has been developed for the determination of the substitution pattern of methyl-starches, -amyloses, -celluloses and -cyclodextrins in the anhydro glucose unit. All eight constituents possible for this type of copolymers could be separated by high-pH anion-exchange chromatography with pulsed amperometric detection (PAD). Peaks were assigned by comparison with synthesized standard compounds. For quantitative evaluation the relative response factors of the O-methyl-glucose derivatives were determined.
引用
收藏
页码：41 / 45
页数：5
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