Self-assembled fluorescent magnetic nanoprobes for multimode-biomedical Imaging

被引:0
作者
Lim, Eun-Kyung [1 ]
Haam, Seungjoo [1 ]
机构
[1] Yonsei Univ, Dept Chem & Biomol Engn, Coll Engn, Seoul 120749, South Korea
来源
NANOTECHNOLOGY 2011: ADVANCED MATERIALS, CNTS, PARTICLES, FILMS AND COMPOSITES, NSTI-NANOTECH 2011, VOL 1 | 2011年
关键词
Nanoprobe; Magnetic resonance imaging; Multimode; Fluorescent; RESONANCE; NANOPARTICLES; CANCER; NANOCRYSTALS; EXOCYTOSIS; PROBES; AGENTS; EPR;
D O I
暂无
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
We fabricated multimode nanoprobes for acquisition of biological information at different object levels, i.e., in vivo detection and ex vivo validation for characterizing tumor angiogenesis. Fluorescent magnetic nanoprobes (FMNPs) were synthesized by using amphiphilic pyrenyl polyethylene glycol (Py-PEG) and superparamagnetic MnFe2O4 nanocrystals (MNCs). Py-PEG, which is synthesized by conjugation of hydrophilic PEG with hydrophobic and fluorescent 1-pyrenebutyric acid through an esterification process, is capable of selfassemblyandmaintaining a highUVfluorescent intensity in aqueous phase. Py-PEG can be used as a fluorescent surfactant that simultaneously and efficiently encapsulates MNCs to exhibit fluorescent andmagnetic properties as well as maintaining highwater-solubility. Consequently,weproved that our biologically non-toxic FMNPs were prominent multimode imaging probes by showing not only excellent MR sensitivity but also high illumination intensity with strong signal strength under short exposure time of UV light from the extensive imaging studies of in vitro/vivo and ex vivo using orthotopic and xenograft mice models.
引用
收藏
页码:359 / 362
页数:4
相关论文
共 23 条
[1]   Fluorescence-modified superparamagnetic nanoparticles:: Intracellular uptake and use in cellular imaging [J].
Bertorelle, Franck ;
Wilhelm, Claire ;
Roger, Jacky ;
Gazeau, Florence ;
Menager, Christine ;
Cabuil, Valerie .
LANGMUIR, 2006, 22 (12) :5385-5391
[2]   CARDIAC EJECTION FRACTION - PHANTOM STUDY COMPARING CINE MR IMAGING, RADIONUCLIDE BLOOD POOL IMAGING, AND VENTRICULOGRAPHY [J].
DEBATIN, JF ;
NADEL, SN ;
PAOLINI, JF ;
SOSTMAN, HD ;
COLEMAN, RE ;
EVANS, AJ ;
BEAM, C ;
SPRITZER, CE ;
BASHORE, TM .
JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING, 1992, 2 (02) :135-142
[3]   In vivo magnetic resonance detection of cancer by using multifunctional magnetic nanocrystals [J].
Huh, YM ;
Jun, YW ;
Song, HT ;
Kim, S ;
Choi, JS ;
Lee, JH ;
Yoon, S ;
Kim, KS ;
Shin, JS ;
Suh, JS ;
Cheon, J .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (35) :12387-12391
[4]   PROTEIN SURFACE INTERACTIONS IN THE PRESENCE OF POLYETHYLENE OXIDE .1. SIMPLIFIED THEORY [J].
JEON, SI ;
LEE, JH ;
ANDRADE, JD ;
DEGENNES, PG .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 142 (01) :149-158
[5]   Near-infrared fluorescent nanoparticles as combined MR/optical imaging probes [J].
Josephson, L ;
Kircher, MF ;
Mahmood, U ;
Tang, Y ;
Weissleder, R .
BIOCONJUGATE CHEMISTRY, 2002, 13 (03) :554-560
[6]   Nanoscale size effect of magnetic nanocrystals and their utilization for cancer diagnosis via magnetic resonance imaging [J].
Jun, YW ;
Huh, YM ;
Choi, JS ;
Lee, JH ;
Song, HT ;
Kim, S ;
Yoon, S ;
Kim, KS ;
Shin, JS ;
Suh, JS ;
Cheon, J .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (16) :5732-5733
[7]   Magnetic fluorescent delivery vehicle using uniform mesoporous silica spheres embedded with monodisperse magnetic and semiconductor nanocrystals [J].
Kim, J ;
Lee, JE ;
Lee, J ;
Yu, JH ;
Kim, BC ;
An, K ;
Hwang, Y ;
Shin, CH ;
Park, JG ;
Kim, J ;
Hyeon, T .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (03) :688-689
[8]  
Lee JH, 2007, NAT MED, V13, P95, DOI [10.1038/nm1467, 10.20659/jfp.13.1_95]
[9]   Dual-mode nanoparticle probes for high-performance magnetic resonance and fluorescence imaging of neuroblastoma [J].
Lee, Jae-Hyun ;
Jun, Young-Wook ;
Yeon, Soo-In ;
Shin, Jeon-Soo ;
Cheon, Jinwoo .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (48) :8160-8162
[10]   Monitoring exocytosis and release from individual mast cells by capillary electrophoresis with laser-induced native fluorescence detection [J].
Lillard, SJ ;
Yeung, ES ;
McCloskey, MA .
ANALYTICAL CHEMISTRY, 1996, 68 (17) :2897-2904